Rhizobium bacteria fix atmospheric nitrogen during symbiosis with legume plants only after bacterial division is arrested. The role of the major vegetative sigma factor, SigA, utilized by Rhizobium bacteria during symbiosis is unknown. By using PCR technology, a portion of the sigA gene corresponding to domain II was directly amplified from Rhizobium etli total DNA by using two primers designed...

To determine the salivary secretory immunoglobulin A (sIgA) response to repeated bouts of unaccustomed, downhill running (eccentrically biased) and examine potential protective immunological adaption from a repeated bout effect. Eleven active but untrained males (age: 19.7±0.4 years; VO2peak: 47.8± 3.6 ml · kg(-1) · min (-1)) performed two 60 min bouts (Run 1 and Run 2) of downhill running (-13...

OBJECTIVE Prior research had shown that an additional training session immediately after acute stress increased release of salivary secretory immunoglobin A (sIgA) in a group trained with 5-day Integrative Body-Mind Training (IBMT) in comparison to a control group given the same amount of relaxation training. However, 5 days of training did not influence the basal secretion of sIgA. The current...

Does the experience of stress during child care lead to downregulation of the immune system, in particular in low-quality care? Saliva was collected from 68 toddlers attending center or family child care at home and at child care, and assayed for secretory IgA (SIgA). Caregiver sensitivity was used as an index of quality of care and was observed during three videotaped episodes of 10 min. Diurn...

Secretory immunoglobulin A (sIgA) antibodies are the first line of defence at the genital mucosa, and are thought to hinder viral infections by binding to conformational epitopes on the viral capsid. To investigate if cervical sIgA binds to conformational epitopes of the Human papillomavirus type 16 (HPV16), cervical mucus samples from 109 HPV16-infected patients were examined in a HPV16 virus-...

Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to those of Mycobacterium smegmatis MysA and MysB proteins, respectively, and also ...

This case-control study was conducted to determine the protective role of salivary secretory immunoglobulin A (SIgA) levels in the unstimulated whole saliva of dental caries active (Group I and II) and caries free children (Group III). Thirty children aged 4-8 years were selected. Their DMFT (Decayed Missing Filled teeth for permanent teeth) and/or df-t (decayed, filled teeth for deciduous teet...

Saliva secretory IgA (sIgA), secretory component (SC); serum immunoglobulins (IgG, IgA, IgM), complement (C3, C4), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) were performed in 32 patients with ankylosing spondylitis and 29 normal controls. They were investigated for carriage in the faeces of Klebsiella spp. on 3 occasions over the previous months. Throat swabs and urine ...