Metal electrodes with micron scale width enable the heating of less than a dozen cells in a confluent layer at predictable temperatures up to 85 °C with an accuracy of ±2 °C. Those performances were obtained by a preliminary robust temperature calibration based on biotin-rhodamine fluorescence and by controlling the temperature map on the substrate through thermal modeling. The temperature accu...

Novel classes of anticancer drugs, including proteasome inhibitors and Hsp90 inhibitors, potently induce heat shock proteins (Hsps). Because Hsps show antiapoptotic activities, we suggested that suppression of such induction may sensitize cancer cells to these drugs. Here, we knocked out the major heat shock transcription factor HSF-1 in several cancer cell lines using small interfering RNA and...

Heat shock proteins (HSPs) have proven to be effective tools for extending invertebrate lifespan, and in C. elegans daf-2 mutants, longevity resulting from loss of insulin/insulin-like signals is at least partly dependent upon elevated HSP expression. In mice, inhibition of the orthologous growth hormone/insulin-like growth factor I (GH/IGF-I) pathway has similar pro-longevity effects. A recent...

Spinally transected rats given legshock whenever one hindleg is extended learn to maintain a flexion response that decreases net shock exposure. Prior exposure to response-independent (noncontingent) shock prevents learning. This behavioral deficit was eliminated by systemic administration of the nonselective opioid antagonist naltrexone (Experiment 1). The deficit was also blocked by intrathec...

This paper investigates the asymmetric impact of energy consumption on economic growth by including oil prices, capital and labour as additional determinants in production function. In doing so, the non-linear ARDL bounds testing approach is applied for the period of 1985QI-2016QIV. The empirical evidence confirms the presence of symmetric and asymmetric cointegration between energy consumption...

Septic shock and multiple organ failure may be associated with coagulation activation, disseminated fibrin formation, and consumption of coagulation inhibitors such as antithrombin 111. We have evaluated prospectively coagulation measurements in patients with severe chemotherapy-induced neutropenia. This group of patients was chosen because of their high risk of developing severe septic cornpli...

clinical syndrome that can be readily diagnosed at the bedside by observing hypotension and signs and symptoms of hypoperfusion (eg, mental status changes, weakness, cyanosis, dyspnea, and oliguria). There is a wide range of causes of shock, but few presentations include jugular venous distention (JVD). The common pathophysiology in the differential diagnosis of shock with JVD (Table) is inhibi...

AIMS This trial was designed to test the hypothesis that shock efficacy during follow-up is not impaired in patients implanted without defibrillation (DF) testing during first implantable cardioverter-defibrillator (ICD) implantation. METHODS AND RESULTS Between February 2011 and July 2013, 1077 patients were randomly assigned (1 : 1) to first time ICD implantation with (n = 540) or without (...

We propose a credit stress testing method that puts together: (1) a bottom-up corporate default prediction model capable of translating shocks to its input variables into the default likelihood of a target portfolio, and (2) a set of stress-testing regressions which relate the presumed adverse macroeconomic scenarios to shocks to the inputs of the default prediction model. This method can produ...