We evaluated the usefulness of enumeration of fecal leukocytes and erythrocytes in making an early diagnosis of Shigella infection, where Shigella is a leading cause of invasive diarrhea. Stool specimens from 561 invasive diarrhea patients were submitted for microscopic examination. A presumptive diagnosis of shigellosis based on microscopic examination was made in 389 of them; 227 had stool cu...

background and objectives: shigellosis is an acute gastroenteritis that is one of the most common causes of morbidity and mortality in children with diarrhea in developing countries. the purpose of this study was to describe the distribution of shigella serogroups and serotypes and their antibacterial drug resistance profiles. materials and methods: fecal samples of all children suffering from ...

OBJECTIVE The epidemiology and antibiotic susceptibility of Shigella species changes over time. Updated susceptibility knowledge is necessary for appropriate empirical antibiotic treatment. Thus, this research aimed to study these changes in 2 time periods with an interval of 10 years. METHODS Two hundreds and three Shigella strains, isolated from stool samples of diarrheic patients at the Ce...

After a useful account of sigmoidoscopy, a short historical survey and a discussion of their geographical distribution, the bacillary, protozoal, and helminthic dysenteries are described in considerable detail. Following this well documented and balanced survey, it is disappointing to have paratyphoid infections and food poisoning dismissed in four pages, especially as it is admitted that parat...

Two colored latex kits (the Wellcolex Colour Salmonella Test [WCT-Salmonella] and the Wellcolex Colour Shigella Test [WCT-Shigella]; Division Diagnostics, Laboratories Wellcome S.A., Paris, France), which allow identification of the most frequently encountered Salmonella serogroups and Shigella species, respectively, were evaluated. WCT-Salmonella and WCT-Shigella yielded sensitivities of 98.4 ...

Shigella flexneri proliferate in infected human epithelial cells at exceptionally high rates. This vigorous growth has important consequences for rapid progression to life-threatening bloody diarrhea, but the underlying metabolic mechanisms remain poorly understood. Here, we used metabolomics, proteomics, and genetic experiments to determine host and Shigella metabolism during infection in a ce...

Five strains of Bacteroides fragilis isolated from human feces were assayed for their inhibitory activities against Shigella flexneri in vitro. Inhibition was not detected when Shigella and the Bacteroides strains were simultaneously inoculated into a defined liquid medium containing glucose. Inhibition was apparent only when Shigella was inoculated into established Bacteroides cultures. In exp...

Shigella, the causative agent of bacillary dysentery invades intestinal epithelial cells using a type III secretion system (T3SS). Through the injection of type III effectors, Shigella manipulates the actin cytoskeleton to induce its internalization in epithelial cells. At early invasion stages, Shigella induces atypical Ca(2+) responses confined at entry sites allowing local cytoskeletal remod...

The Shigella bacteria cause bacillary dysentery, which remains a significant threat to public health. The genus status and species classification appear no longer valid, as compelling evidence indicates that Shigella, as well as enteroinvasive Escherichia coli, are derived from multiple origins of E.coli and form a single pathovar. Nevertheless, Shigella dysenteriae serotype 1 causes deadly epi...