Double mutants of Escherichia coli dam (DNA adenine methyltransferase) strains with ruvA, ruvB, or ruvC could not be constructed, whereas dam derivatives with recD, recF, recJ, and recR were viable. The ruv gene products are required for Holliday junction translocation and resolution of recombination intermediates. A dam recG (Holliday junction translocation) mutant strain was isolated but at a...

DNA adenine methylase (dam) mutants of Salmonella enterica serovar Typhimurium grown under laboratory conditions express the std fimbrial operon, which is tightly repressed in the wild type. Here, we show that uncontrolled production of Std fimbriae in S. enterica serovar Typhimurium dam mutants contributes to attenuation in mice, as indicated by the observation that an stdA dam strain is more ...

[1] The relative impact of a large upstream dam versus in‐reach groundwater pumping on stream temperatures was analyzed for humid, semiarid, and arid conditions with long dry seasons to represent typical climate regions where large dams are present, such as the western United States or eastern Australia. Stream temperatures were simulated using the CE‐QUAL‐W2water quality model over a 110 kmmod...

In 1891 a report was published by an ASCE committee to investigate the cause of the Johnstown flood of 1889. They concluded that changes made to the dam by the South Fork Fishing and Hunting Club did not cause the disaster because the embankment would have been overflowed and breached if the changes were not made. We dispute that conclusion based on hydraulic analyses of the dam as originally b...

In this study, stochastic seismic response of a rock-fill dam is investigated by finite element method. The Keban dam constructed in Elazığ, Turkey is chosen as a numerical example. The interaction of the rock-fill dam with the reservoir is neglected, but not the foundation rock. The properties of the dam materials were taken from the dam project and assumed to be linearly elastic, homogenous a...

We have used the Koppes and Nordstrøm (Cell 44:117-124, 1986) CsCl density transfer approach for analysis of DNA from exponentially growing, isogenic Escherichia coli dam+ and dam mutant cells to show that timing between DNA replication initiation events is precise in the dam+ cells but is essentially random in the dam cells. Thus, methylation of one or more GATC sites, such as those found in u...

We compared the bioaccumulation of lead (Pb), cadmium (Cd), zinc (Zn), copper (Cu), nickel (Ni) and iron (Fe) with antioxidant enzyme activity in tissues of the snails, Lymnaea natalensis, exposed to elements of two differently polluted dams. 45 snails were exposed to sediment and water collected from Wight Dam (reference) whilst another 45 snails were also exposed to sediment and water collect...

BACKGROUND The placement of rubber dam has the potential to alter the airflow through nasal and oral cavities. Pediatric dentist should be aware whether the use of a rubber dam affects the oxygen saturation (SpO2) in children. To assess the effect of rubber dam on arterial blood SpO2 in children of 6-12 years age. MATERIALS AND METHODS Totally, 60 ASA Class I patients of 6-12 years age, rando...

Deoxyadenosine methyltransferase (Dam) methylates the deoxyadenine residues in 5'-GATC-3' sequences and is important in many cellular processes in Escherichia coli. We performed a computational analysis of the entire E. coli genome and confirmed that GATC sequences are distributed unevenly in regulatory regions, which suggests that Dam might regulate gene transcription. To test this, a high-den...

The mismatch repair system of Escherichia coli K12 removes mispaired bases from DNA. Mismatch repair can occur on either strand of DNA if it lacks N6-methyladenines within 5'-GATC-3' sequences. In hemimethylated heteroduplexes, repair occurs preferentially on the unmethylated strand. If both strands are fully methylated, repair is inhibited. Mutant (dam-) strains of E. coli defective in the ade...