Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell components in phage assembly haven't been well understood. It was previously reported that two unidentified proteins were present in bacteriophage Sf6 virion (Casjens et al, 2004, J.Mol.Biol. 339, 379-394, Fig. 2A). Using tandem mass spectrometry, we have identified the two proteins as outer membrane...

X-ray diffraction and Raman spectroscopy were employed to study the phase diagram of SF6 at pressures up to 32GPa. The known phase transformation of SF6 phase I to phase II at around 2GPa was confirmed. Discontinuities in the pressure dependence of the Raman frequencies and positions of X-ray reflections indicate further phase transitions at 10(1) and 19(1)GPa. The crystal structures of phases ...

We report on Si nanopatterning through an on-chip self-assembled porous anodic alumina (PAA) masking layer using reactive ion etching based on fluorine chemistry. Three different gases/gas mixtures were investigated: pure SF6, SF6/O2, and SF6/CHF3. For the first time, a systematic investigation of the etch rate and process anisotropy was performed. It was found that in all cases, the etch rate ...

Many viruses, including bacteriophage, have the inherent ability to utilize several types of proteinaceous receptors as an attachment mechanism to infect cells, yet the molecular mechanisms that drive receptor binding have not been elucidated. Using bacteriophage Sf6 and its host, Shigella flexneri, we investigated how Sf6 utilizes outer membrane protein A (OmpA) for infection. Specifically, we...

[1] Dispersion of the tracer sulphur hexafluoride (SF6) during the Southern Ocean Iron Enrichment Experiment (SOIREE) provided an estimate of vertical exchange at the base of the surface mixed layer (60 m) at 61 S 140 E. Budget analysis confirmed that the SF6 patch was well constrained by surface mapping, with the decline in total SF6 showing good agreement with that predicted from wind speed p...

Methane emission f rom l ivestock and agricultural wastes contribute globally more than 3 0 % to the anthropogenic atmospheric methane source. Estimates of this number have been derived f rom respiration chamber experiments. W e determined methane emission rates f rom a tracer experiment in a modern cow shed hosting 43 dairy cows in their accustomed environment. During a 24-hour period the conc...

Agitated saline contrast echocardiography is often used to determine blood flow through intrapulmonary arteriovenous anastomoses (Q̇IPAVA). We applied indicator dilution theory to time-acoustic intensity curves obtained from a bolus injection of hand-agitated saline contrast to acquire a quantitative index of contrast mass. Using this methodology and an in vitro model of the pulmonary circulatio...