We describe a receptor assay of digoxin activity involving Na+/K+-ATPase (EC 3.6.1.37) derived from human heart tissue. The procedure requires 500 microL of serum or plasma and incorporates one purification step, with Sep-Pak C18 cartridges (Waters). The method appears to be considerably more specific for digoxin and its cardio-active metabolites than are conventional immunoassays. Cardio-inact...

We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule fr...

1. Pratt, J. J., Steroid immunoassay in clinical chemistry. Clin. Chem. 24, 1869 (1978). 2. Shackleton, C. H. L., and Whitney, J. 0., Use of Sep-pak cartridges for urinary steroid extraction: Evaluation of the method for use prior to gas chromatographic analysis. Clin. Chim. Acta (in press). 3. Thenot, J.-P., and Horning, E. C., MOTMS derivatives of human steroids for GC and GC-MS studies. Anal...

A high-performance liquid chromatographic procedure with a postcolumn fluorescence derivatization is developed for the analysis of oleandrin in bovine blood. Oleandrin is separated by an octadecylsilane-bonded column with a mobile phase containing dehydroascorbic acid. The effluent of the column is mixed with concentrated hydrochloric acid and passed through poly(tetrafluoroethylene) tubing mai...

BACKGROUND Zebrafish is an important model organism for human neurobehavioural studies and compound screening for neurodegenerative disorders like Alzheimer's disease and dementia. PURPOSE We wanted to analyse the rapid neurobehavioural effects based on acetylcholinesterase inhibitors from Tephrosia purpurea in zebrafish. METHODS Tephrosia purpurea (a herbal neuroactive molecule) extract wa...