this experiment was conducted to evaluate the addition of seminal plasma to coated spermatozoa after 72 h. semen was collected from four taleshi rams by artificial vagina. to prepare coated spermatozoa, semen was collected in tube containing tris-fructose-15% egg yolk. after initial evaluation, the samples were mixed and centrifuged. after removal of supernatant and dilution, samples were divid...

Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established t...

background: the trace element copper has been identified as a highly toxic element for sperm. it is known to affect sperm motility in humans and experimental implantation of copper in the epididymis vas deferens and scrotum of mammals has been demonstrated to affect fertility detrimentally. objective: sperm concentration motility vitality and morphology are parameters used to evaluate potential...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO),...

Follistatin is a binding protein for the activin and inhibin family of hormones, regulating their biological activity. In the male reproductive tract, the interaction of these factors is likely to be involved in the regulation of the proliferation of several cell types. We have investigated the presence of follistatin and activin A in seminal plasma using specific immunoassays and have localize...

Apart from its obvious role in transporting male gametes, seminal plasma provides a protective environment for ejaculated spermatozoa and improves their fertilizing potential. Compositional changes can alter these properties and cause reproductive disorders. Thus, characterization of seminal plasma has the potential to provide biomarkers of reproductive disorders in males. Seminal plasma is a h...

Efavirenz (EFV) is one of the most commonly prescribed antiretrovirals for use in the treatment of human immunodeficiency virus (HIV) infection. EFV is extensively metabolized by cytochrome P450 to a number of oxygenated products; however, the pharmacologic activity and distribution of these metabolites in anatomic compartments have yet to be explored. The systemic distribution of EFV oxidative...

Ejaculated ram spermatozoa, freed from seminal plasma by a dextran/swim-up procedure and exposed to cold shock, were incubated with ram seminal plasma proteins and analyzed by fluorescence markers and scanning electron microscopy. Seminal plasma proteins bound to the sperm plasma membrane modified the functional characteristics of damaged spermatozoa, reproducing those of live cells. Scanning e...