The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen store...

The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred an...

Artificial insemination (AI) using chilled ram semen has not been adopted in Uruguay yet, probably due to a lack of information about the use of this technology in the country. This study evaluated the fertility rate obtained in Merino ewes inseminated with ram semen stored at 5 °C in a TRIS-based extender in Uruguayan field conditions. The effect of storage period of semen (Experiment 1) and d...

Studies were conducted using 68 males from the seventh generation of a line of meat-type breeder chickens selected for increased duration of fertility of frozen-thawed semen and 69 males of the randomly selected control line. These chickens were used to determine the presence or absence of differences between the selected and control lines with respect to fertility and spermatozoal motility whe...

In humans, use of frozen semen is still limited to the activities of a few centers. Procedure for freezing semen involves adding .1 ml of glycerol for each milliliter of semen to be frozen. The specimen is then transferred into sterilized ampules in 1 ml quantities and sealed. The ampules are then suspended in liquid nitrogen vapor at a temperature of -99 degrees C for 1/2 hour. Following t...

OBJECTIVE To determine why patients with cancer stop storing semen in a sperm bank program. DESIGN Retrospective study. SETTING Hospital andrology laboratory. PATIENT(S) Cancer patients (n = 56) who discontinued sperm storage. INTERVENTION(S) A database of 342 patients with cryopreserved sperm was searched for disease diagnosis, marital status before and after diagnosis, type of therapy...

The Punjab Urial (Ovis vignei punjabiensis) is an endangered subspecie of ovidae, distributed as small scattered populations in the forest belt of the Himalayan foothills of Pakistan and in the areas enclosed by the Indus and the Jhelum rivers. The present study was conducted to evaluate the liquid storage of Punjab Urial spermatozoa in different extenders for use in future in situ conservation...