نتایج جستجو برای: selectable marker
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The fission yeast Schizosaccharomyces pombe is an attractive model organism for studying eukaryotic biology due to the ease of genetic and molecular manipulations (3). In recent years, several selectable markers and promoters have been developed to enhance our ability to manipulate gene expression in this yeast. Recently, Invitrogen (Carlsbad, CA, USA) has developed the SpECTRA S. pombe Expres...
To date, the insect nodavirus flock house virus (FHV) is the only virus of a higher eukaryote that has been shown to undergo a full replicative cycle and produce infectious progeny in the yeast Saccharomyces cerevisiae. The genome of FHV is composed of two positive-sense RNA segments: RNA1, encoding the RNA replicase, and RNA2, encoding the capsid protein precursor. When yeast cells expressing ...
Allelic replacement in the Burkholderia genus has been problematic due to the lack of appropriate counter-selectable and selectable markers. The counter-selectable marker sacB, commonly used in gram-negative bacteria, is nonselective on sucrose in many Burkholderia species. In addition, the use of antibiotic resistance markers of clinical importance for the selection of desirable genetic traits...
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