background: production of tissue plasminogen activator protein (t-pa) in prokaryotes systems has many problems such as the lack of active protein production, multiple purification steps, and renaturation process which has been shown to be costly and time-consuming. methods: in this study, reteplase which is the nonglycosylated active domain of t-pa was used to transform top10 escherichia coli (...

Abstract Objective Snails secrete different types of mucus that serve several functions, and are increasingly being exploited for medical cosmetic applications. In this study, we explored the protein pattern compared biological properties secreted from mantle collar foot two snail species, Lissachatina fulica Hemiplecta distincta . Result Protein profile showed a between species secretory parts...

  Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...

Light-driven engineered microalgal cultivation in photobioreactors coupled with membrane separations of secreted metabolites as next generation sustainable bio-processes.

SCUBE-1 is a newly identified, secreted cell surface protein that determined during early embryogenesis. This consists of cascading EGF-like repeats following the N-terminal signal peptide sequence, spacer region, cysteine-rich repeat motifs, and CUB domain at C-terminus. These molecules are stored in alpha granules inactivated platelets, translocated to platelet after activation by thrombin, a...

A cell line expressing the CD2v protein of ASFV was generated. The efficient expression determined by immunofluorescence and Western blotting. Ni-affinity purified from supernatant cultures. CD2v-expressing cells showed properties hemadsorption, secreted exhibited hemagglutinating activity. antigenicity immunoprotection ability were evaluated immunizing pigs alone, combined with a cell-line-exp...