نتایج جستجو برای: sau3ai
تعداد نتایج: 94 فیلتر نتایج به سال:
Experiments were designed to allow isolation of Streptomyces coelicolor promoters that depend on the whiG sporulation gene, which encodes a putative sigma factor important in the sporulation of aerial hyphae. The strategy, based on earlier evidence that sigma WhiG is limiting for sporulation (K. F. Chater, C. J. Burton, K. A. Plaskitt, M. J. Buttner, C. Méndez, and J. Helmann, Cell 59:133-143, ...
A 55-kDa outer membrane protein of Porphyromonas gingivalis W50 is a significant target of the serum immunoglobulin G antibody response of periodontal disease patients and hence may play an important role in host-bacterium interactions in periodontal disease. The gene encoding the 55-kDa antigen (ragB, for receptor antigen B) was isolated on a 9.5-kb partial Sau3AI fragment of P. gingivalis W50...
بیماری فیلودی یکی از بیماری¬های مهم فیتوپلاسمایی در پروانش می¬باشد. طی سال 1391 به منظور شناسایی این بیماری در استان ماندران از فضای سبز و پارک های شهرستان¬ بهشهر استان مازندران بازدید گسترده¬ای به عمل آمد. پس از مشاهده بوته¬های دارای علائم فیلودی در شهرستان بهشهر؛ این بوته¬ها به گلخانه تحقیقاتی موسسه تحقیقات فارس منتقل شدند و عامل بیماری با پیوند به بوته¬های سالم پروانش و با سس به بوته¬های سال...
بیماری اسکراپی در گوسفندان، بیماری تحلیل برنده نورونهای عصبی است که به دلیل تاخوردگی غیرمعمول پروتئین پریون در مغز، که باعث ایجاد شکل مقاوم این پروتئین می‌شود، بوجود می‌آید. فاکتورهای ایمنولوژیکی و ژنتیکی علل عمده ایجاد کننده این اسکراپی ذکر شدند. از جمله این فاکتورهای ژنتیکی که ممکن است در ایجاد و یا پیشرفت بیماری نقش داشته باشند، وجود چندشکلی کدون شماره 171 از ژن پروتئین پریون می‌باشد. هدف از...
Genus and Species. Bos indicus and Bos taurus. Locus Name. Bovine stearoyl-coenzyme A desaturase (SCD). Source and Description of Primers. Primers were designed that amplified a 135-bp region in SCD exon 6. Sequences from human (GenBank accession number Y13647), rat (AB003357), mouse (M21285), and pig (Z97186) were aligned to identify a conserved region in which to design the primers. The forwa...
Polymorphism. Two polymorphic microsatellite loci, BFRO010 and BFRO011, were identified in genomic DNA of grayling (Thymallus thymallus). Source and Description. The template DNA was isolated from a library of size-selected (200 to 800 bp) Sau3AI genomic restriction fragments from grayling. The DNA fragments were cloned into pBluescript II SK+ (Stratagene, La Jolla, CA) vector, previously restr...
Twenty-two infectious bursal disease virus (IBDV) strains were examined using the reverse transcriptase/polymerase chain reaction-restriction endonuclease (RT/PCR-RE) assay. A 394-bp fragment of the VP2 gene was amplified and tested for six different restriction enzyme sites. Although the EcoRII enzyme was used in previous RT/PCR-RE assays, results obtained using the isoschizomer BstNI were mor...
BACKGROUND The accumulation of mutagenic substances in the human body may result in DNA metabolism disruption followed by carcinogenesis. As a consequence of mutations in the genes coding for transmembrane protein pumps, the intracellular concentration of xenobiotics may significantly increase. This, in turn, may provoke altered risk for cancer development. The gene known to be the most relevan...
Twenty-five unique CfoI-generated whole-cell DNA profiles were identified in a study of 30 Paenibacillus alvei isolates cultured from honey and diseased larvae collected from honeybee (Apis mellifera) colonies in geographically diverse areas in Australia. The fingerprint patterns were highly variable and readily discernible from one another, which highlighted the potential of this method for tr...
The gene coding for the flavodoxin protein from Desulfovibrio vulgaris (Hildenborough) has been identified, cloned, and sequenced. DNA fragments containing the flavodoxin gene were identified by hybridization of a mixed synthetic heptadecanucleotide probe to Southern blots of SalI-digested genomic DNA. The nucleotide sequences of the probe were derived from the published protein primary structu...
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