Because oxygen intermediates secreted by inflammatory leukocytes are postulated to play a role in potentiating carcinogenesis, we investigated the ability of macrophages to induce oxidative DNA damage in eukaryotic cells. Murine macrophages, obtained from sites of inflammation and stimulated with 12-O-tetradecanoylphorbol-15-acetate, induced the formation of 5,6-ring-saturated thymine bases in ...

Because oxygen intermediates secreted by inflammatory leu kocytes are postulated to play a role in potentiating carcinogenesis, we investigated the ability of macrophages to induce oxidative DNA damage in eukaryotic cells. Murine macrophages, obtained from sites of inflammation and stimulated with 12-0tetradecanoylphorbol-15-acetate, induced the formation of 5,6ring-saturated thymine bases in t...

We investigate the effect of galactic winds on the Lyα forest in cosmological simulations of structure and galaxy formation. We combine high resolution N-body simulations for the evolution of the dark matter with a semi-analytic model for the formation and evolution of galaxies which includes detailed prescriptions for the long-term evolution of galactic winds. We find that the main statistical...

a proposed method for protecting carbon steel and hence reducing iron release in an aqueous solution saturated with h2s is presented in this paper. the method is based on forming a stable and resistant sulfide layer (pyrite, fes2, or pyrrhotite, fe(1-x)s, phase or a mixture of both) on the carbon steel surface. the study on the formation of the sulfide layer was carried out considering the effe...

Proteolytic studies of rat liver metallothionein reconstituted in vitro with Cu salts revealed that the 2 metal centers fill in an ordered fashion. The B cluster in the NH2-terminal beta domain fills prior to Cu binding in cluster A. This is in contradistinction to cluster formation induced by the binding of Cd or Zn ions in which cluster A is the center of initial binding. The formation of met...

Fatty acid-induced lipotoxicity plays a critical role in the pathogenesis of nonalcoholic liver disease. Saturated fatty acids and unsaturated fatty acids have differential effects on cell death and steatosis, but the mechanisms responsible for these differences are not known. Using cultured HepG2 cells and primary mouse hepatocytes, we found that unsaturated and saturated fatty acids different...

By measuring its IR phosphorescence the formation of singlet molecular oxygen O2 photosensitized by rhodamine dyes is directly proved. The O2 formation rate is compared with that expected from the low probability (≈1%) of intersystem crossing of the photosensitizers. The quantum yield for triplet population and the triplet lifetime of the investigated dyes is measured by using a laser-scanning-...