Traditionally studies of cold tolerance in insects have focused on seasonal adaptations related to overwintering that are observed after weeks or months of exposure to low temperature. In contrast, an extremely rapid cold-hardening response was observed in nonoverwintering stages that confers protection against injury due to cold shock at temperatures above the supercooling point. This response...

Effects of long day and short day treatments during the embryonic and larval stages on induction of pupal diapause were studied on a diapausing race of Sarcophaga peregrina. Two long day (15L 9D) cycles during 2 days before or after the larviposition completely stopped the induction of pupal diapause on larvae which grow in short day condition before and after the long day treatment. The sensit...

Forensically important entomological specimens recovered from 95 forensic cases of human cadavers from April 1993 to May 1996 in Malaysia were identified and analysed. The results indicated that 73.7% of these specimens were Chrysomya species, occurring either as single or mixed infestations. Of these, the most prominent species were Ch megacephala (F.) and Ch rufifacies (Macquart). Other fly m...

Storage-protein mRNA was found to be abundant in poly(A)-containing RNA extracted from the fat-body of third-instar larvae of Sarcophaga peregrina (fleshfly). This RNA sedimented at the position of 19S on sucrose-density-gradient centrifugation and the product of its translation in vitro was 75K protein (protein of mol.wt. 75 000), which was precipitated specifically with antibody against stora...

ABSTR ACT: Intestinal myiasis is a clinical condition when the fly larvae inhabit the gastrointestinal tract and are excreted in feces. A 26-year-old young male visited the outpatient clinic of Manipal Teaching Hospital, Nepal, with symptoms of loose stool, bloating of abdomen, abdominal discomfort, intestinal hurry following meals, and passage of live wriggling maggots in the stool. Macroscopi...

Cilia and flagella are rare in nongerminal tissues of anthropods, and are generally thought to be restricted to sperm and sensory cells in insects (2). Whitten (5) has reported the presence of kinetosomes at the base of mitotrichia in the dipteran fly Sarcophaga bullata, but reports no evidence of the organization of fibrous elements characteristic of cilia and or flagella. During an ultrastruc...

Insect oocyte nuclei contain different extrachromosomal nuclear bodies including Cajal bodies and interchromatin granule clusters (IGCs). In the present study, we describe IGC equivalents in the vitellogenic oocytes of the flesh fly, Sarcophaga sp. These structures were found to consist of 20-40-nm granules and also include the fibrillar areas of high and low electron density. Immunogold labeli...