As the gold standard of protein detection, enzyme-linked immunosorbent assay (ELISA) is widely used in medical treatment and biology. Here, we report a digital ELISA method that combines fluorescence-coded magnetic beads with micropore arrays to effectively improve accuracy detection. Fluorescence coded were as solid support ELISA, which modified specifically capture IL-6 serum, then combined g...

OBJECTIVE To produce the monoclonal antibodies (mAbs) against hygromycin B phosphotransferase (HPT) and to develop immunoassay based on mAbs for biosafety assessment of HPT in genetically modified rice (GM rice). METHODS BALB/c mice were immunized with purified recombinant 6His. HPT protein, and the conventional hybridoma technology was used to generate the monoclonal hybridoma cells. ELISA a...

Rift Valley fever (RVF) virus is an important zoonotic and a potential biothreat agent. This paper describes validation of sandwich and capture enzyme-linked immunoassays (ELISA) based on gamma-irradiated antigens for the detection of RVFV-specific IgG and IgM antibody in humans. Validation data sets derived from testing field-collected sera from Africa (n=2400) were dichotomised according to t...

BACKGROUND Malaria control efforts are limited in rural areas. A low-cost system to monitor response without the use of electricity is needed. Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. A three-part system using ELISA was developed consisting of a microfluidic chip, hand crank centrifuge, and a smartphone. METHODS A circular...

Five different immunoassay formats were examined for their ability to detect a minute quantity of prey remains in predator guts. The convergent lady beetle, Hippodamia convergens Guérin-Meneville, that had consumed either one or five pink bollworm, Pectinophora gossypiella (Saunders), eggs was evaluated by the following immunoassays: three variations of enzyme-linked immunosorbent assay (ELISA)...

Kallikrein-related peptidases (KLKs) have been proposed as potential cancer biomarkers. Kallikreinrelated peptidase 5 (KLK5) is a secreted trypsin-like protease of the KLKs. Until now, detection of KLK5 in both biological fluids and tissues has been described frequently due to the potential of being a new cancer biomarker. Our objective was to prepare KLK5 antibodies and establish an ELISA meth...

hydatidosis, caused by the larval stage of echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. as its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. sera (250 samples) were collected from 90 cases of hydatidosis pro...

Direct, indirect and double antibody sandwich methods of ELISA were examined for their ability to detect and discriminate between granulosis viruses from Pieris brassicae, Agrotis segetum and Cydia pomonella and for their specificity in the presence of host material. The indirect method was the most sensitive, capable of detecting down to about I ng of dissolved capsules/ml compared with IO ng/...