An asd-deleted (Deltaasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype ...

ABSTRACT: Pullorum disease is described worldwide and caused by Salmonella enterica subspecies serovar Gallinarum biovar (S. Pullorum). S. infection important in commercial poultry, provoking a systemic with high mortality rates. Its occurrence requires notification, when it diagnosed breeding flocks, its eradication demanded. The aim of this study was to report severe outbreak young Guinea fow...

ALL TYPES of poultry are important reservoirs of paratyphoid infections. As applied to poultry diseases, the term ''paratyphoid" denotes diseases caused by micro-organisms of the Salmonella group other than Salmonella pullorum (puUorum disease) and Salmonella gallinarum (fowl typhoid). More than 60 of the 200-odd types of Salmonella that are known to exist cause diseases in poultry. With the ra...

Musca domestica L. (Diptera: Muscidae), the housefly, exhibits unique immune defenses and can produce antibacterial substances upon stimulation with bacteria. On the basis of the cDNA library constructed using the suppression subtractive hybridization method, a 1188-bp antibacterial substance gene, which we named AS566, was amplified by rapid amplification of cDNA ends from M. domestica larva s...

In this study, 2 bacteriophages of Salmonella Pullorum were isolated using an enrichment protocol and the double agar layer method. They were named PSPu-95 and PSPu-4-116, respectively, against clinical isolates of Salmonella Pullorum SPu-95 and SPu-116. The host ranges of the 2 bacteriophages were determined by performing spot tests with 20 bacteria strains. Both bacteriophages had wide host r...