Background and Objective: Salmonellosis is an important infectious zoonotic disease that makes it even more significant to identify and control the causative strains. Molecular methods, especially polymerase chain reaction (PCR), for virulence genes can help to quickly and accurately identify Salmonella strains. Accordingly, the purpose of this study was molecular identification based on sivH, ...

Dry heat was used to inactivate Salmonella newington, Salmonella typhimurium, Salmonella anatum, Salmonella kentucky, Salmonella cubana, Salmonella seftenberg, Salmonella thompson, and Salmonella tennessee in corn flour at 10 and 15% moisture. The flour was spray inoculated at 10(5) Salmonella cells per g and then stored at 49 degrees C (120 degrees F); viable Salmonella cells were counted on T...

Salmonella enterica serovar Typhimurium and S.enterica serovar Enteritidis are the most frequently isolated serovars from food-borne diseases throughout the world. According to their antigenic profiles, salmonella shows different disease syndromes and host specificities. It is necessary and important to discriminate salmonella serovars from each other in order to ensure that each pathogen and i...

AIM This study was carried out to determine the prevalence, distribution, and identification of Salmonella serotypes in diarrheagenic infants and young animals, including sewage waste and fresh vegetables. MATERIALS AND METHODS A total of 550 samples were processed for the isolation of Salmonella spp., using standard microbiological and biochemical tests. Further polymerase chain reaction (PC...

BACKGROUND In sub-Saharan Africa, non-typhoidal Salmonella (NTS) are emerging as a prominent cause of invasive disease (bacteremia and focal infections such as meningitis) in infants and young children. Importantly, including data from Mali, three serovars, Salmonella enterica serovar Typhimurium, Salmonella Enteritidis and Salmonella Dublin, account for the majority of non-typhoidal Salmonella...

Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Salmonella bongori (V), and were clustered into 17 groups by their comparison pa...

Salmonella is one of the most important zoonotic pathogens with many virulence factors playing a major role in its pathogenesis. The aims of this study were to detect spvA, int2 and invC virulence genes of different Salmonella serotypes isolated from various clinical animal and human sources, and to investigate antibiotic resistance patterns among these serotypes. Using a PCR assay, a total of ...

the effect of triton x-100, na cholate and tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of salmonella typhimurium was studied. the detergents were used in various concentrations (1.6 to 64 mm) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. results showed that no inne...