Figure S1. Schematic illustration of puhA-zif268 DNA construct S2 Figure S2. Agarose gels showing amplification and assembly of the puhA-zif268 construct. S3 Figure S3 Sequence confirmation of puhA-zif268 construct by MWG Eurofins S3 Figure S4 SDS-PAGE and absorption spectra of purified WT and zfRC-LH1 S4 Figure S5 MALDI-TOF for the WT and zfRC-LH1 S5 Figure S6 MALDI-TOF mass spectra of DNA-cyc...

1. Experimental methods 2. Strains and plasmids used in this study (Table S1) 3. Deduced functions and sequence comparison of the tar genes (Table S2) 4. Schematic diagram of the tar gene cluster direct cloning (Figure S1) 5. Restriction mapping of pCAP01-tar and its derivatives (Figure S2). 6. Results of heterologous expression of the tar gene cluster in S. coelicolor M1146 (Figure S3) 7. Resu...

The nucleotide sequence of the operon encoding maize chloroplast ribosomal protein genes S7 and S12 and the promoter activity of a chimeric construct of the -10/-35 sequence of this operon (attached to a promoterless chloramphenicol acetyltransferase gene) have been determined. This operon occurs in the chloroplast genome divided in two parts: part A contains exon 1 of rpS12 (encoding the N-ter...

The filamentous fungus Podospora anserina presents a degeneration syndrome called Senescence associated with mitochondrial DNA modifications. We show that mutations affecting the two different and interacting cytosolic ribosomal proteins (S7 and S19) systematically and specifically prevent the accumulation of senDNA alpha (a circular double-stranded DNA plasmid derived from the first intron of ...

lac promoter mutations which help to define the degree of overlap between the promoter and operator have been isolated and characterized. lacPr115 partially relieves the dependence of lac expression on the catabolite gene activator protein. P'11.5 is an A/T + T/A transversion at base-pair + 1 (the site of transcription initiation in vitro) that has no effect on the repressor-operator interactio...

Photoinduced cross-linking of Escherichia coli 70 S ribosomes with [3H]puromycin has led to the labeling of ribosomal proteins S7, S14, S18, L18, and L29. Proteolytic fragmentation of these proteins and separation of the peptide mixtures by C18 reversed-phase high performance liquid chromatography resulted in six puromycin-labeled peptides which were applied to sequence analysis. The following ...

Table S1. Crystal data and structure refinement.......................................... ....................................S2 Figure S1. Powder EPR spectra of 1•2[Al(ORF)4] at various temperatures (120-170K)..............................S3 Figure S2. Powder EPR spectra of 1•2[Al(ORF)4] at various temperatures (190-290K)... ...........................S4 Figure S3. Powder EPR spectrum of 1•2[A...

Possibilities and realization of system for control of more entry doors and ventilation in industry based on using wireless computer network technology are considered and described in the paper. Described solution has possibility to monitor, control and perform diagnostic of system using wireless computer network. The system is consisted of set of sensors, actuators and control devices (PLCs). ...

Tables S3 to S7 as zipped archive 1131898-TableS3-7.zip