The formation of stem cell-derived tumors (teratomas) is observed when engrafting undifferentiated embryonic stem (ES) cells, embryoid body-derived cells (EBCs), or mammalian embryos and is a significant obstacle to stem cell therapy. We show that in tumors formed after engraftment of EBCs into mouse brain, expression of the pluripotency marker Oct-4 colocalized with that of prostate apoptosis ...

Fbw7 is a tumor suppressor often deleted or mutated in human cancers. It serves as the substrate-recruiting subunit of a SCF ubiquitin ligase that targets numerous critical proteins for degradation, including oncoproteins and master transcription factors. Cyclin E was the first identified substrate of the SCFFbw7 ubiquitin ligase. In human cancers bearing FBXW7-gene mutations, deregulation of c...

The ability to maintain a proportionate body plan in individuals of different sizes is essential for proper development. Despite wide interest, little is known about the mechanisms underlying scaling with size. I will discuss our efforts to reveal the molecular basis for scaling in different systems. A general ‘‘Expansion– Repression’’ feedback topology will be presented, which ensures scaling ...

A large perfect crystal interferometer with two interference loops offers novel applications in neutron interferometry. Coherent beam manipulation with special prism and phase shifter arrangements is discussed and prism materials that are able to preserve beam coherence in case of thicker prisms are investigated. A set of four identical prisms has been used to determine the vertical coherence f...

Adv. Mater. 2020, 32, 1906641 In the originally published version of this article, data for histological evaluation CPDs toxicity in brain at CON-7d was duplicated Figure S18, instead correct image CPDs-1d being used. The figure is hereby corrected. authors apologize mistake and any inconvenience caused.

BACKGROUND Most plasmids replicate only within a particular genus or family. METHODOLOGY/PRINCIPAL FINDINGS Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Pe...

The trinucleotide AUG was condensed at the 5'-end with N-bromoacetyl-p-aminophenylphosphate. This bromoactylated AUG analog reacted irreversibly with the mRNA binding site of Escherichia coli 70S ribosomes. After reaction of 70S ribosomes with the AUG analog, labeled 30S subunits could be isolated that were programmed for initiation-factor-dependent binding of fMet-tRNAfMet. This shows that thi...

A primer set of oligonucleotides (S18 and S19) from the ompC gene of Salmonella has been evaluated for specific detection of Salmonella by polymerase chain reaction (PCR). This primer set successfully amplified 40 Salmonella serovars (60 isolates), but not 24 non-Salmonella bacteria (42 isolates) that have been tested so far. The uniqueness of these primer sequences was also confirmed. The sens...

Relative quantification of 40 nucleic acid sequences by multiplex ligationdependent probe amplification. Nucleic Acids Res 2002;30:e57. 30. Xiao W, Oefner PJ. Denaturing high-performance liquid chromatography: a review. Hum Mutat 2001;17:439–74. 31. Cha RS, Thilly WG. Specificity, efficiency, and fidelity of PCR. PCR Methods Appl 1993;3:S18–29. 32. Dehainault C, Lauge A, Caux-Moncoutier V, Page...

In BHK cells infected with pseudorabies virus, there was a substantial increase in the phosphorylation of ribosomal protein S6. This increase occurred between 2 and 4 h after infection and persisted at least until 9 h. We estimated that in mock-infected cells S6 contained, on an average, one phosphate group per protein chain, whereas in infected cells this rose to between four and five phosphat...