The double exponential model t t M T Be Ae vl μ μ − − + = was fitted to log10(vl) data from day 0 to day 28 post ART. When vl was undetectable it was replaced with the limit of detection (80 copies per ml), indicated by the dashed line in Figure S1 and S2. Fits were performed by nonlinear least squares regression, confidence intervals were calculated by bootstrapping the cases and trimming the ...

There are several downstream analysis goals for which RNA-seq is well suited. Main categories of these are described briefly below with reference to supporting materials. Refer to S2 Table for specific tools relevant to many of these areas. For each application, a basic data recommendation is provided. It is important to remember that these are simply examples. In addition to the varying demand...

PURPOSE The large number of deaths in a short period of time due to the spread of severe acute respiratory syndrome (SARS) infection led to the unparalleled collaborative efforts world wide to determine and characterize the new coronavirus (SARS-CoV). The full genome sequence was determined within weeks of the first outbreak by the Canadian group with international collaboration. As per the Wor...

The main aim of this study was to obtain the Crimean-Congo hemorrhagic fever virus (CCHFV) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made CCHFV glycoprotein in a mouse model. We designed and synthesized a codon-optimized G1/G2 gene from the G1 and G2 parts of the CCHF...

Comment on reversibility We and others have observed differences in the reversibility measured by the QPatch instrument and by manual patch clamp: washout of lipophilic compounds is delayed in the QPatch. The probable reason for this is the presence of unrecorded cells within the chip. One major difference as compared to manual patch clamp is that QPatch does not allow continuous flow of extrac...