A total of 125 Russian Red Pied cows were genotyped for the prolactim-related gene. The PRL-RsaI genotypes were analysed using the Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) method. In this breed, the frequencies of alleles were as follows;  A= 0.794 and B= 0.206. The frequencies of AA, AB and BB genotypes were 0.598, 0.392 and 0.01; respectively. Results sho...

Polymorphism. Two alleles of a DNA region (HFABPL) with high homology to the bovine heart-fatty acid binding protein (H-FABP) cDNA were detected through RFLP analysis of PCR products digested with the restriction enzyme RsaI. Primer Source. Primers were designed based on the bovine cDNA sequence for H-FABP (Billich et al., 1988) to amplify between nucleotides 66-676. The primer s quences were ...

In this study, we selected 58 styrene-exposed workers, measured personal styrene exposure, evaluated genotypes relevant drug-metabolizing enzymes (CYP2E1, EPHX1, GSTM1 and GSTT1) which may explain the variability in the urinary metabolite excretion. The results showed that, in different levels of styrene exposure groups, there is a significant association between urinary metabolites and some ge...

Hepatitis C virus isolates which disclosed a novel genotype 1-associated restriction pattern by restriction fragment length polymorphism analysis were characterized. Except for a mother and child pair, the patients were unrelated. Sequence analysis showed a G-->A substitution leading to a new RsaI recognition site. Phylogenetic analysis revealed that these isolates constitute a novel genetic li...

To establish a possible role of genomic imprinting in the carcinogenesis of epithelial ovarian cancer, we determined the imprinting status of both IGF-II and H19 genes in 43 ovarian cancers, 7 low malignant potential ovarian tumors, and their matched normal tissues. In ovarian cancer, loss of heterozygosity (LOH) of IGF-II, H19 RsaI, and H19 AluI was found in 4 of 24 (16.7%), 3 of 20 (15%), and...

The bovine prolactin (PRL) and bovine growth hormone (bGH) genes exhibit several polymorphisms. Some of them can be detected by molecular techniques using restriction endonucleases, such as RsaI for the PRL gene and MspI for the bGH gene. We examined the relationship between the PRL-RsaI and bGH-MspI polymorphisms and some economically important characteristics of Holstein cows. Research was co...

A study on the occurrence of Aspergillus section Nigri species on grapes from four traditional grape-producing areas in Greece during the 2011/2012 vintage, and their capability to produce OTA was conducted. One hundred and twenty-eight black aspergilli isolates were characterised at the species level initially by the use of morphological criteria in accordance with appropriate keys, followed b...

New primer-enzyme combinations for terminal restriction fragment length polymorphism (T-RFLP) targeting of the 16S rRNA gene were constructed by using the T-RFLP analysis program (designated TAP T-RFLP) located at the Ribosomal Database Project website, and their performance was examined empirically. By using the fluorescently labeled 516f primer (Escherichia coli positions 516 to 532) and 1510...