Locusta migratoria is a classic hemimetamorphosis insect and has caused widespread economic damage to crops as a migratory pest. Researches on the expression pattern of functional genes in L. migratoria have drawn focus in recent years, especially with the release of genome information. Real-time quantitative PCR is the most reproducible and sensitive approach for detecting transcript expressio...

Variability within eight cpDNA introns including trnS-trnfM, trnL-trnT, trnH-psbA, trnF-trnL, trnD-trnT, trnCrpoB, rps16 and matK, and the nuclear waxy introns was examined in seven species of Capsicum (C. annuum, C. baccatum, C. chinense, C. frutescens, C. pubescens, C. chacoense and C. rhomboideum) in order to evaluate the feasibility of utilizing these loci for DNA barcoding within the C. an...

A weed plant of the species Lupinus polyphyllus was found in settlements located on territory natural park “Vepssky forest” Leningrad region. The is known as a transforming southern regions Russia. distribution L. within Vepsian forest occurs not due to migration north along transport routes, one might assume, but flight from gardens and formation spontaneous invasive populations. goal study id...

The importance of intronic sequences for expression of the mouse ribosomal protein gene rpL32 was evaluated by transfection experiments with a series of mutant constructs in which one or more of the three rpL32 introns was totally or partially deleted. When transiently transfected into monkey kidney (COS) cells or stably transfected into mouse L cells, a mutant that lacked all three introns was...

This study uses phylogenetic relationships of the species-rich section Astragalus (Incani), to follow up on recent evidence pointing rapid and plant diversification patterns in west Iran. Section Incani is introduced for its taxonomic complication resulting from overlapping morphological characters, but few studies have been done this section; hence, we also lack a robust time-calibrated chrono...

Quantitative real time PCR (qRT-PCR) has emerged as a reliable and reproducible technique for studying gene expression analysis. For accurate results, the normalization of data with reference genes is particularly essential. Once the transcriptome sequencing of Frankliniella occidentalis was completed, numerous unigenes were identified and annotated. Unfortunately, there are no studies on the s...

Nuclear and cytoplasmic DNA barcoding regions are useful for plant identification, breeding, phylogenesis. In this study, the genetic diversity of 17 Diplotaxis species, was investigated with 5 barcode markers. The allelic variation based on sequences chloroplast markers including spacer between trnL trnF tRNA-Phe gene (trnL-F), rubisco (rbcl), maturase K (matk), as well internal transcribed (I...

Reticulate evolution is characterized by occasional hybridization between two species, creating a network of closely related taxa below and at the species level. In the present research, we aimed to verify the hypothesis of the allopolyploid origin of hexaploid C. album s. str., identify its putative parents and estimate the frequency of allopolyploidization events. We sampled 122 individuals o...