Paeonia ostii ‘Feng Dan’ is an economically important, multipurpose woody plant in terms of its medical, ornamental, and oil values; however, there a noticeable contradiction between the increasing demands lack excellent germplasm resources because traditional breeding propagation approaches. In vitro embryo culture attractive option for this issue. This study presents protocol immature P. Dan’...

A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini, an important rootstock in Pistachio orchards in Iran. In present study, the efficiency of a new medium called GNH (Garoosi, Nezami and Haddad) was investigated against some standard media. Different vitamins, calcium (Ca) sources and plant growth regulators (PGRs) were tested on in vitro shoot multiplication and ...

Salvia tomentosa is a perennial sage of the maquis vegetation, strongly aromatic, with medicinal and ornamental value. Aiming to facilitate promotion species in floriculture sector, its micropropagation was studied terms effect explant origin medium plant growth regulators on vitro cultures establishment, shoot proliferation rooting. Shoot tip or single node explants excised from either grown s...

fusarium wilt caused by fusarium oxysporum f.sp. lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. in order to increase the tolerance to this disease, a triple structure containing pr1, chitinase and glucanase genes controlled by 35s promoter was transferred to tomato. eight days after planting on pre-culture medium, exp...

Dehydrin is known to have an important role in plant response and adaptation abiotic stresses including drought high salinity. Previous research reported the isolation of full-length coding sequence (CDS) DHN1 from sugarcane var. PSJT 941, it shares a homology with DHN genes sorghum other varieties. In this study, CDS was cloned under constitutive CaMV35S promoter transformed into calli mediate...

Abstract The paper presents a technique for micropropagation of endangered in Europe and extinct Poland Pulsatilla vulgaris ex situ conservation the genetic resources. Genotype-dependent induction somatic embryogenesis rooting was revealed series two experiments (I II) performed under same experimental conditions. Shoot tips seedlings were best explants both Murashige Skoog (MS) medium suppleme...

Fusarium wilt caused by Fusarium oxysporum f.sp. Lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. In order to increase the tolerance to this disease, a triple structure containing PR1, chitinase and glucanase genes controlled by 35S promoter was transferred to tomato. Eight days after planting on pre-culture me...

the aim of this research was to evaluate the influence of rooting cofactors including hydrogen peroxide, boric acid and thiamine along with indole-3-butyric acid (iba) on rooting of olive cuttings cv. rashid in autumn 2007 and spring 2008. the study was carried out as a factorial experiment in a completely randomized design (crd) with three replications, and five cuttings in each replication. r...

Ficus benjaminais a very popular and resistant indoor plant in temperate areas. Cutting is the easiest propagation method of this plant and it requires special treatments such as auxin and appropriate rooting medium. In order to test the suitable auxin (IBA) concentration and rooting media, a factorial experiment carried out based on randomized complete block design with three replications. Exp...

Micropropagated plants are usually cultured in in vitro environments, with a high relative humidity and low light intensity, taking nutrients and energy from the culture medium. During acclimatization, physiological and structural changes allow micropropagated plants to adapt to the new environmental conditions, mainly to low relative humidity and high light intensity. As a result, plants becom...