An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained...

Presence of pharmaceutically important alkaloids in various parts of the plant body made Rauvolfia serpentina an overexploited one and led to its inclusion in Red Data Book of India as an endangered species. Conventional propagation is beset with problems like poor seed viability, low seed germination rate and delayed rooting of cuttings. In vitro clonal propagation may therefore be beneficial ...

In vitro plantlet regeneration has been obtained from 20-25days old seedling shoot tip segments of Cucumis melo (L.). We were examined using various phyto hormones individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP (1.0-3.0 mg/L) Kn (1.0-3.0 mg/L),IAA (0.5 mg/L)+ BAP (1.0-3.0 mg/L) and IAA (0.5 mg/L)+Kn (1.0-3.0 mg/L) for multiple shoots prolifer...

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obta...

and inoculation with the arbuscular mycorrhizal fungi Glomus mosseae (Nicol. and Gerd.) Gerdmann and Trappe, Glomus intraradices Schenck and Smith or Glomus viscosum Nicolson on shoot apical growth of plantlets that had been micropropagated from MM 106 apple (Malus pumila L.) and Mr.S. 2/5 plum (Prunus cerasifera Ehrh.) rootstocks. Unfertilized and non-mycorrhizal plantlets showed no apical gro...

Ratoon sugarcane plantlets in southern China have suffered a serious chlorosis problem in recent years. To reveal the causes of chlorosis, plant nutrition in chlorotic sugarcane plantlets and the role of manganese (Mn) in this condition were investigated. The study results showed that the pH of soils growing chlorotic plantlets ranged from 3.74 to 4.84. The symptoms of chlorosis were similar to...

Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability to metabolize N(6)-benzyladenine (BA). Formation of N(6)-benzyladenosine-5'-mo...

he forage legume cicera chickling (Lathyrus cicera (L.) D.C.) disributed in Mediterranean region is gaining importance in terms f economy and agriculture in Turkey. However, full potential of he legume has to be realized yet due to the presence of neuotoxin, -N-oxalyl-l-a, -diaminopropionoc acid (ODAP) causing athyrism. This study aimed to develop efficient micropropagation ystem using longitud...

Seed characteristics and in vitro culture of C. tamala embryos were studied. Embryos desiccated below 50% (fresh weight) exhibited poor morphogenetic response in vitro and confirmed the recalcitrant nature of seeds. The immature embryos of various developmental ages (4-16 week after flowering, WAF) were cultured on different strengths of MS medium. Morphogenesis responses were recorded after 10...

An efficient protocol was achieved for rapid propagation of Wedelia chinensis (Osbeck) Merr. through axillary bud proliferation and ex vitro rooting. Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 8.87 microM) and indole-3-butyric acid (IBA; 2.46 microM) was optimal for axillary bud proliferation, which developed a mean of 8.3 shoots/node. Excision and culture of node segm...