نتایج جستجو برای: rk39 antigen

تعداد نتایج: 200325  

Journal: :Journal of clinical pathology 2003
P Salotra G Sreenivas K R Beena A Mukherjee V Ramesh

AIMS To evaluate the sensitivity and specificity of serological, immunohistochemical, and molecular methods in the diagnosis of post kala-azar dermal leishmaniasis (PKDL). METHODS Twenty five patients with confirmed PKDL and 25 controls were included in the study. G2D10, a monoclonal antibody against Leishmania, was used for the immunohistochemical (IHC) staining of lesion sections to visuali...

Journal: :iranian journal of immunology 0
durria mansour laboratory of biomedical research, ahfad university for women, p.o. box 167, omdurman, sudan elfadil m. abass laboratory of biomedical research, ahfad university for women, p.o. box 167, omdurman, sudan abdelhafeiz mahamoud laboratory of biomedical research, ahfad university for women, p.o. box 167, omdurman, sudan abdallah el harith retired microbiologist, wijngaard 155, 8212 cj, lelystad, the netherlands

background: until now, the comparison of the rk39 strip test (rkt) and direct ag-glutination test (dat) for detection of visceral leishmaniasis (vl) is exclusively based on either positive or negative qualification of the reaction outcome. objective: in this study, we compared the diagnostic performance of rkt and dat for vl both qualitatively and semi-quantitatively. methods: for comparison ba...

2014
Epco Hasker Paritosh Malaviya Kamlesh Gidwani Albert Picado Bart Ostyn Sangeeta Kansal Rudra Pratap Singh Om Prakash Singh Ankita Chourasia Abhishek Kumar Singh Ravi Shankar Mary E. Wilson Basudha Khanal Suman Rijal Marleen Boelaert Shyam Sundar

INTRODUCTION Asymptomatic persons infected with the parasites causing visceral leishmaniasis (VL) usually outnumber clinically apparent cases by a ratio of 4-10 to 1. We assessed the risk of progression from infection to disease as a function of DAT and rK39 serological titers. METHODS We used available data on four cohorts from villages in India and Nepal that are highly endemic for Leishman...

2016
Vidya Nand Ravi Das Ravindra Nath Pandey Niyamat Ali Siddiqui Lloyd A C Chapman Vijay Kumar Krishna Pandey Greg Matlashewski Pradeep Das

BACKGROUND Visceral Leishmaniasis (VL) is a neglected tropical disease that afflicts some of the poorest populations in the world including people living in the Bihar state of India. Due to efforts from local governments, NGOs and international organizations, the number of VL cases has declined in recent years. Despite this progress, the reservoir for transmission remains to be clearly defined ...

Journal: :The Southeast Asian journal of tropical medicine and public health 2001
S K Sharma M L Das S Rijal S P Sah S Koirala

We report a case of exclusive involvement of lymph node in leishmaniasis presenting as generalized lymphadenopathy. The diagnosis of lymphatic leishmaniasis was confirmed by the presence of Leishmania donovani body in fine needle aspiration cytology, positive direct agglutination test and anti-rK39 antibodies. The bone marrow aspiration was negative for Leishmania donovani body. This is the fir...

ژورنال: :medical laboratory journal 0
مهدی فخار m fakhar assistant professor of parasitology, department of parasitology and mycology, molecular and cellular biology research centerدانشگاه علوم پزشکی مازندران،ساری،ایران احسان احمدپور e ahmad pour phd student of parasitology, department of parasitology and mycoloگروه انگل شناسی و قارچ شناسی، دانشگاه علوم پزشکی مازندران،ساری،ایران

چکیده لیشمانیوز احشایی (کالاآزار) نوعی بیماری عفونی سیستمیک است که روش های تهاجمی بیوپسی طحال، کبد ویا آسپیراسیون مغز استخوان بعنوان روش استاندارد طلایی برای تشخیص آن تعیین شده اند. در حال حاضر روشهای تشخیصی غیر تهاجمی مختلفی با ویژگی و حساسیت های متفاوت برای تشخیص کالاآزار وجود دارد. دراین میان روش سرولوژی آگلوتیناسیون مستقیم (dat) به شرط تهیه آنتی ژن مطلوب، روش مناسبی بوده و به آسانی و در شرا...

2009
Elizabeth Castro Moreno Andréa Vieira Gonçalves Anderson Vieira Chaves Maria Norma Melo José Roberto Lambertucci Antero Silva Ribeiro Andrade Deborah Negrão-Corrêa Carlos Mauricio de Figueiredo Antunes Mariângela Carneiro

BACKGROUND One of the most important drawbacks in visceral leishmaniasis (VL) population studies is the difficulty of diagnosing asymptomatic carriers. The aim of this study, conducted in an urban area in the Southeast of Brazil, was to evaluate the performance of serology to identify asymptomatic VL infection in participants selected from a cohort with a two-year follow-up period. METHODOLOG...

2014
Marleen Boelaert Kristien Verdonck Joris Menten Temmy Sunyoto Johan van Griensven Francois Chappuis Suman Rijal

BACKGROUND The diagnosis of visceral leishmaniasis (VL) in patients with fever and a large spleen relies on showing Leishmania parasites in tissue samples and on serological tests. Parasitological techniques are invasive, require sophisticated laboratories, consume time, or lack accuracy. Recently, rapid diagnostic tests that are easy to perform have become available. OBJECTIVES To determine ...

Journal: :Journal of clinical microbiology 2004
D Otranto P Paradies M Sasanelli R Spinelli O Brandonisio

An rK39 immunochromatographic test and immunofluorescent-antibody test (IFAT) for serodiagnosis of canine leishmaniasis were evaluated. The two tests showed correlation for all but one of the sera obtained from 68 dogs confirmed as leishmaniasis cases and 40 dogs (22 healthy dogs and 18 dogs with other diseases) from areas where the disease is not endemic. Specificity was 100% for both tests, w...

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