' Several perplexing questions involving standardization techniques surface when ultraviolet spectrophotometry is used for the determination of unknown ribonucleic acids isolated from tissue sources. One must consider the effect of hydrolysis on total absorbance, nucleotide makeup of a ribonucleic acid versus molar absorptivity and additivity of riucleotides on total measured absorbance. Experi...

Human peripheral lymphocytes are stimulated to change, in vitro, from a resting state to one of progressive enlargement, culminating in mitosis, by treatment with Phaseolus vulgaris phytohemagglutinin. The sedimentation characteristics and fate of rapidly synthesized RNA have been studied in this system after 20 hours of stimulation with phytohemagglutinin, a time when RNA synthesis has been ac...

We have measured the pyrimidine nucleotide contents of the culture fluid, acid-soluble fraction, and acid-insoluble fraction of cultures of hamster embryo fibroblasts (third subculture) through the final two divisions of growth in culture. The cells show a growth delay between the penultimate and ultimate division periods and a concomitant biochemical synchrony of pyrimidine metabolism. The cel...

The ability of proflavine to directly inhibit protein synthesis has led to an investigation of complex formation between the drug and ribonucleic acid. Evidence has been obtained from absorption and fluorescence spectra, as well as heat denaturation curves, that proflavine forms a complex with transfer ribonucleic acid which is qualitatively similar to the deoxyribonucleic acid-proflavine compl...

PURPOSE To perform an exploratory characterization of repeated sprint sequences (RSS) and repeated acceleration sequences (RAS) in top level soccer referees. METHODS 7 field and 7 assistant referees were monitored during 2007 America's Soccer Cup with GPS technology. Sprints of >18 km·h(-1) and accelerations of >1.5 m·s(-2) were considered as high intensity activities. RSS and RAS were define...

Active 30S and 50S ribosomal subunits were isolated from Bacillus subtilis. These subunits were able to perform not only protein synthesis in the presence of artificial or natural messenger ribonucleic acid but also the specific functions characteristic of each of the subunits. Thus the 30S subunits alone are able to bind formyl-methionyl-transfer ribonucleic acid, and the 50S subunits carry th...

INTRODUCTION ........ ...... ........ 261 GROWTH OF METHANOGENS 262 Techniques for Growth of Methanogens ..... 262 Principal Media......6..3.. . .... ....... .. . 263 Strain Histories.................2..6..4............. 264 Deoxyribonucleic Acid Base Composition Determination (Moles Percent Guanine plusCytosine). 264 GENERATION OF 16S RIBOSOMAL RIBONUCLEIC ACID OLIGONUCLEOTIDE CATALOGS ..........