A deficit of mitochondrial energy metabolism may play a role in migraine pathogenesis. We found in a previous open study that high-dose riboflavin was effective in migraine prophylaxis. We now compared riboflavin (400 mg) and placebo in 55 patients with migraine in a randomized trial of 3 months duration. Using an intention-to-treat analysis, riboflavin was superior to placebo in reducing attac...

Riboflavin is known to be decomposed by specific bacteria. Foster (1,2) showed that the ribityl portion of riboflavin was oxidized by living cells of Pseudomonas ribo$avina, leaving the heterocyclic ring, 7, %dimethylalloxazine (lumichrome), intact. Later, Hou (3) found that lumichrome accumulated as riboflavin disappeared in a culture of tubercle bacilli to which the vitamin had been added. Th...

PURPOSE To evaluate the importance of complete epithelial removal before riboflavin-ultraviolet-A (UVA) corneal collagen crosslinking therapy. SETTING School of Optometry and Vision Sciences, Cardiff University, Wales, United Kingdom. METHODS Riboflavin eyedrops were applied at 5-minute intervals for 35 minutes to the anterior corneal surface of 36 porcine eyes (12 with no epithelial trauma...

The effects of riboflavin on the formation of 6,7-dimethyl-8-ribityllumazine (DMRL) were examined using the growing cells of a riboflavin, adenine deficient-mutant, Bacillus subtilis. The administration of low concentrations (50-500 ng/mL) of riboflavin to the basal medium significantly inhibited the formation of DMRL without any change in growth. Especially, the supplementation of more than 25...

a potent antagonist of riboflavin in the rat, has been found to be nonspecific in the inhibition of the growth of Walker rat Carci noma 256. 7-Ethyl-8-methyl-10-(r-D-ribityl)isoalloxazine (7ethyl-8-methyIflavin) and 7-methyl-8-ethy 1-10-(1' -D-ribityl) isoalloxazine (7-methyl-8-ethylflavin) have been found to replace riboflavin for the growth of and the efficiency of utilization of food by the ...

The effect of riboflavin deficiency on in vivo incorporation of [1-14C] leucine into proteins of liver has been studied on rats following protein depletion and subsequent repletion. The increase in the incorporation of [14C] amino acid into liver proteins follow ing protein depletion was less marked in riboflavin-deficient rats. But repletion with 40% protein demonstrated marked diminished prot...

Purpose To investigate riboflavin concentration on enzymatic resistance following corneal cross-linking (CXL). Methods Ninety-six porcine eyes were divided into five groups in two treatment runs. Group 1 remained untreated. Group 2 received riboflavin 0.05%, group 3 riboflavin 0.1%, group 4 riboflavin 0.2%, and group 5 riboflavin 0.3%. Treated eyes underwent CXL with ultraviolet A at 9 mW/cm2...

FASS, STEVEN, AND RICHARD S. RIVLIN. Regulation of riboflavinmetabolizing enzymes in ribuj?auin de_ficiency. Am, J. Physiol. 217(4) : 988-99 1. 1969.-The effects of riboflavin deficiency in rats on the hepatic activities of flavokinase, FAD pyrophosphorylase, and FMN phosphatase, three enzymes involved in riboflavin metabolism, have been measured and compared to the hepatic concentrations of FA...

A rapid and precise homogeneous enzyme-linked competitive binding assay for riboflavin (vitamin Bz) is described. The method utilizes a malate dehydrogenase/3-carboxymethylriboflavin conjugate in conjunction with soluble riboflavin binding protein. In the absence of the vitamin, the catalytic activity of the enzyme/riboflavin conjugate is inhibited up to 71% by the binding protein. In the prese...

This work shows that the ribC wild-type gene product has both flavokinase and flavin adenine dinucleotide synthetase (FAD-synthetase) activities. RibC plays an essential role in the flavin metabolism of Bacillus subtilis, as growth of a ribC deletion mutant strain was dependent on exogenous supply of FMN and the presence of a heterologous FAD-synthetase gene in its chromosome. Upon cultivation ...