Bacterial strains and growth conditions. Fast-growing soybean Rhizobium strains USDA 191, USDA 192, USDA 193, USDA 194, USDA 201, USDA 205, USDA 206, USDA 208, USDA 214, USDA 217, and USDA 257 were isolated from soil or nodules collected in the PRC (20). Slow-growing soybean rhizobium strains PRC 005, PRC 74, PRC 113-2, PRC121-6, PRC 2031, and PRC B15, were oHtained from T. S. Hu, Institute of ...

Growth and survival of two rhizobial strains, Rhizobium leguminosarum bv. trifolii, and Mesorhizobium ciceri, were used to evaluate residues from the cork industry as potential carriers for production of rhizobial inoculants. A peat-based carrier was used as a control. 41 days after inoculation, the number of viable bacteria was high, about 109 bacteria g-1 for all carriers. Rhizobial survival ...

Previously, we have shown that the nodE gene is a major determinant of the difference in host range between Rhizobium leguminosarum biovars viciae and trifolii. A new genetic test system for stringent functional analysis of nodE genes was constructed. By testing chimeric nodE genes constructed by the exchange of polymerase chain reaction (PCR)-generated restriction cassettes, we show that a cen...