Ten strains of Aeromonas hydrophila isolated from water samples were characterized by various methods including biochemical and physiological tests, as well as PCR-RFLP analysis with Universal 16s rRNA primers. Biochemical characterization and PCR-RFLP analysis proved to be useful, rapid and reliable method for identifying widely distributed Aeromonas spp. from environmental samples. The signif...

Tunga penetrans (L.) and T. trimamillata Pampiglione et al. are the unique species of the genus parasiting men. While morphological identification of gravid females is possible, the diagnosis on non-gravid females, males or body fragment is quite difficult, time consuming and often requires taxonomic experience. In this paper we present a protocol for the diagnosis of these species based on the...

Outer membrane proteins (OMPs) are the major virulent factors of Haemophilus parasuis. PCR-RFLP targeting the ompA gene was conducted to investigate the possibility of genotyping H. parasuis in this study. Fifteen reference strains and 49 isolates from pig farms in northwest China were genotyped by PCR-RFLP with a pair of specific primers. The results indicated that both the 15 reference strain...

BACKGROUND/AIM The purpose of the present study was to determine the distribution and epidemiological features of mycobacteria with molecular methods. MATERIALS AND METHODS Fifty-five culture-positive samples were analyzed by polymerase chain reaction-restriction enzyme length polymorphism (PCR-RFLP) at species level, and their molecular typing was performed with spoligotyping. The IS6110 reg...