The results of molecular genetic analysis 13 polymorphic variants genes, according to the data genome-wide association studies (GWAS) associated with development addictive states, in group persons alcohol dependence (n = 142) and comparison 211) are presented. main genotyping method is polymerase chain reaction (PCR) followed by high resolution melting (HRM) clustering profiles; validated using...

Bovine tuberculosis caused by Mycobacterium bovis remains a significant disease of farmed cattle in many countries despite ongoing tuberculosis eradication programs. Molecular typing methods such as restriction fragment length polymorphism (RFLP) analysis and spoligotyping have been used to identify related herd breakdowns in an attempt to identify more precisely the route of infection into cat...

Assessing microbiological quality assurance by monitoring bacteria in various sources of freshwater used for human consumption, recreation, and food preparation is important for a healthy life. Bacterial number and their community structure in freshwater should be determined as quickly as possible, and "real-time" and "on-site" microbiological methods are required. In this study, we examined th...

Among 12 clarithromycin-resistant Helicobacter pylori strains isolated in Guri, Korea, 8 showed an adenine to guanine mutation at position 2143 (formerly A2144G or E. coli 2059) in the 23S rRNA gene by the PCR-restriction fragment length polymorphism (RFLP) method. The remaining 4 strains, digested by neither BsaI nor BbsI, showed a thymine to cytosine mutation at position 2182 (T2182C) by dire...

Introduction: Dicrocoeliasis is a common disease of bile ducts and gallbladder of domestic and wild ruminants. This disease is caused by different species of dicrocoelium including Dicrocoelium dendriticum. The aim of this study was to identify pathological damages and molecular features associated with this parasite in ruminants. Materials and Methods: In this cross-sectional study, 180 fresh...

Rabies is widespread in all provinces of Iran. This study presents an investigation by a Polymerase Chain Reaction (PCR) technique for the purpose of rabies diagnosis and also Restriction Fragment Length Polymorphism (RFLP) for determination of the type of the rabies virus. In addition, PCR proves to be a powerful tool for molecular epidemiology studies allowing analysis of the infecting strain...

Biochemical polymorphism study is a method of determination of genetic variation. This variability could be a basis for selection and subsequent genetic improvement in farm animals. The polymorphism in the intron 1 of chicken growth hormone (cGH) gene was investigated in the Iranian native fowls by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. The...

Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than f...