The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...

AIM The present study was conducted to investigate the effect of species, breed and age on bacterial load in fresh and frozen semen of Cattle and Buffalo bull. MATERIALS AND METHODS Present study covered 56 cow and 10 buffalo bulls stationed at Central Semen Station Anjora, Durg (Chhattisgarh). Impact of breeds on bacterial load in semen was assessed using six breeds of cattle viz. Sahiwal, G...

The freezability of semen collected during the low (May-July) and the peak (September-November) breeding seasons from 18 Nili-Ravi buffalo bulls of young (3-4 years), adult (6-8 years) and old ( 1215 years) age groups was studied. After extension in lactose.:..fructose-egg-yolk-glycerol extender, semen samples were frozen and stored in liquid nitrogen. Post-thaw motili�y and liveability of sper...

the aim of this study was to determine the effects of the antioxidants superoxide dismutase (sod),glutathione peroxidase (gpx) and butylated hydroxytoluene (bht) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. ejaculates were collected from five holsteinbulls and pooled at 37°c. the semen samples were diluted with a cey extender containing addit...

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...

The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...

the effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°c was evaluated. semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. semen samples were pooled and divided into 4 parts. each part was diluted with...