in vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. micropropagation using nodal segments of rosa canina under different combinations of bap, ga3 and naa on ms and vs, medium was investigated. the results showed that the highest shoot proliferation was obtained on vs medium cont...

rose is considered as one of the most popular and important ornamental plants in the world. during the last decade, tissue culture, micropropagation as well as genetic engineering have prevailed in rose production industry. gene transfer to embryogenic callus, derived from different types of explants is currently one of the most popular methods of improvement of rose cultivars, presented in par...

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NAA on MS and VS, medium was investigated. The results showed that the highest shoot proliferation was obtained on VS medium cont...

Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented withdifferent concentrations of N6-benzyladenine (BA) (0.5, 1, ...

alstroemeria (alstroemeriaceae family) is an ornamental monocot mainly produced for its cut flowers. embryogenic calluses have been employed for gene transformation in alstroemeria. this research work was undertaken to study the embryogenic callus induction in alstroemeria cv. fuego. the vegetative explants (node, internode, leaf) along with various concentrations of auxins (picloram, naa, iaa,...

Tissue culture provides a useful system to investigate how plant hormones are involved in this process. Auxin and cytokinin are widely used in plant regeneration. Gibberellin is also an important plant hormone in regulating plant growth and development. It is interesting to know the effects of gibberellin and its signalling pathway on plant regeneration. In this report Arabidopsis thaliana land...

Intraspecific fusion of protoplasts with nuclei in Antrodia cinnamomea lengthened the time for development of colonies on regeneration medium and reduced the number of protoplasts capable of regeneration. When 2.1 × 10 C98 protoplasts were fused with C96 nuclei, 1750 colonies appeared on the regeneration medium, and 742 of them were putative nuclear hybrids as their colonies were different from...

Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...

Among the diverse alkaliphilic Bacillus strains, only a little have been reported to be genetically transformed. In this study, an efficient protoplast transformation procedure was developed for recalcitrant alkaliphilic Bacillus sp. N16-5. The procedure involved polyethylene glycol-induced DNA uptake by the protoplasts and subsequent protoplast regeneration with a developed hard agar regenerat...

We performed an in vitro study in order to determine possible triggers of hair cell regeneration in the chick basilar papilla following degeneration. We compared the response of sensory epithelium damaged by collagenase treatment with that damaged by acoustic trauma. The former exhibited no proliferative activity, but the latter did. The basilar papillae damaged by acoustic trauma could have pr...