The present study evaluates immunogenicity and protection potency of a codon-optimized GRA1 DNA vaccine, wild type GRA1 DNA vaccine and an adjuvanted recombinant GRA1 protein vaccine candidate in BALB/c mice against lethal toxoplasmosis. Of the three GRA1 vaccines tested, the recombinant GRA1 protein vaccine results reveal significant increase in immune response and prolonged survival against a...

Background:  With one-third of the world’s population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should s...

background: recently, botulinum neurotoxin (bont)-derived recombinant proteins have been suggested as potential botulism vaccines. here, with concentrating on bont type e (bont/e), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of bont serotypes a, b and e binding subdomains, and a monovalent recombinant protein, which contains...

brucellosis is a debilitative disease that imposes costs on both economy and society. it is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. in iran, brucella melitensis is a common causative agent for brucellosis and bp26 protein of this bacterium having a good antigenesity and an important vaccine candidate. in this study b. meli...

Modified vaccinia virus Ankara (MVA) has become a vaccine vector of choice for recombinant vaccine development. A MVA-based rabies vaccine would be advantageous for use as a vaccine for dogs (and wildlife), particularly if it proves innocuous and efficacious by the oral route. Here, the generation and immunological testing of a recombinant MVA expressing a rabies virus glycoprotein gene is desc...

green plants have emerged as ideal platforms for production of recombinant vaccine during recent decades. various antigens relating to a large number of animal and human diseases have been studied in different plant species for production of recombinant vaccines. despite the unique advantages of plant systems as green factories for production of recombinant vaccines, there are some major hurdle...

bordetella pertussis is a gram negative bacterium that causes respiratory tract infection in human (whooping cough). pertussis toxin (pt) is the main component of current acellular pertussis vaccine and the s1 (subunit1) is the main immunogenic part of it. thus, s1 has been the target of many studies as a potent candidate of acellular vaccine against bordetella pertussis, lacking the side effec...

conclusions these findings of the present study indicate that the recombinant coree1e2 glycoprotein is effective in inducing neutralizing antibodies, and is an influential hcv vaccine candidate. results in our study, the virus-like particle of rcoree1e2 with 70 nm size, was shown by electron microscopy and proved the self-assembly in vitro in a yeast expression system. materials and methods by ...

background: the aim of present study was to evaluate the protective efficacy of live recombinant l. tarentolae expressing kmp11-ntgp96-gfp fusion as candidates for live engineered recombinant vaccine against visceral leishmaniasis in balb/c mice. methods: kmp-11 and nt-gp96 genes cloned into the pjet1.2/blunt cloning vector and then into pegfp-n1 expression vector. the kmp-11, nt-gp96 and gfp f...

background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...