background: the over-expression of recombinant proteins in large amount is important for production of therapeutic proteins and structural study. there are several systems for expression of recombinant proteins. one of the most relevant expression systems is escherichia coli (e. coli). although this organism has many advantages, most of recombinant proteins expressed in e. coli hosts form inclu...

BACKGROUND Serum antibodies against major outer membrane protein (MOMP) and heat shock protein 60 (HSP60) from Chlamydia trachomatis are correlated with sequelae following infection. Since bacterial and human HSP60 share considerable sequence homology, cross-reactivity to human HSP60 is suggested as being involved in tubal factor infertility (TFI). The aim was to investigate whether antibodies ...

background: the purity and correct folding of a recombinant protein is critical for any structural, biochemical and vaccine design studies. plasmodium vivax duffy binding protein-ii is a leading vaccine candidate for vivax malaria. in the present study, the purification process of recombinant dbp-ix (a variant form of pvdbp-ii) was optimized to achieve the highest yield and purity. moreover, na...

The envelopes of elementary bodies of Chlamydia spp. consist largely of disulfide-cross-linked major outer membrane protein (MOMP) and two cysteine-rich proteins (CRPs). The MOMP gene of Chlamydia psittaci 6BC has been sequenced previously, and the genes encoding the small and large CRPs from this strain were cloned and sequenced in this study. The CRP genes were found to be tandemly arranged o...

Specific and sensitive amplification of major outer membrane protein (MOMP) gene (ompA) DNA sequences of Chlamydia species with various MOMP genotypes was achieved by a two-step polymerase chain reaction (PCR). Degenerate, inosine-containing oligonucleotide primers homologous to the 5' and 3' ends of the translated regions of all chlamydial MOMP genes were used in a PCR to amplify a DNA fragmen...

HLA class I-restricted CD8+ CTLs specific for the major outer membrane protein (MOMP) of Chlamydia trachomatis are present in the peripheral blood of humans who acquired genital tract infections with the organism. Three HLA-A2-restricted epitopes and two HLA-B51-restricted epitopes were identified in serovar E-MOMP. One of the five epitopes spans a variable segment of MOMP and is likely a serov...

background: the witches’ broom disease of lime caused by candidatus phytoplasma aurantifolia, is the most devastating disease of acidian lime in the southern parts of iran. objectives: at present, no effient method has been developed for controlling the disease, therefore quarantine approaches such as early detection and subsequent eradication of infected trees is very important. toward this ...

The major outer membrane protein (MOMP) of C. trachomatis is surface exposed and accounts for 60% of the outer membrane protein of this widespread pathogen. This protein has been shown to contain conserved regions among the 15 serovars of C. trachomatis as well as variable domains (VD) that are partially responsible for antigenic differences among the serovars. In addition antibodies raised to ...

Recently, we have shown that a vaccine consisting of a purified preparation of the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP) and Freund's adjuvant can protect mice against a genital challenge. Here, we wanted to determine if CpG motifs could be used as an immune modulator to the MOMP to induce protection in mice against an intranasal (i.n.) challenge. On...

Thirty-six ruminant isolates of Chlamydia psittaci, previously classified as invasive or non-invasive in a mouse model of virulence, were compared by analysing AluI restriction patterns of the major outer-membrane protein (MOMP) gene after DNA amplification by the polymerase chain reaction. The 24 invasive isolates, although from various origins, all belonged to serotype 1 and represented a str...