A critical step in the turnover of yeast mRNAs is decapping. Two yeast proteins, Dcp1p and Dcp2p, are absolutely required for decapping, although their precise roles in the decapping reaction have not been established. To determine the function of both Dcp1p and Dcp2p in decapping, we purified recombinant versions of these proteins from Escherichia coli and examined their properties. These expe...

Enantioselective production of (S)-2-phenyl-1-propanol is important as in order to be applied industry, a high degree optical purity required. Besides organocatalysts and metal complexes, biocatalysts can used for its synthesis their isolated form or whole-cell biocatalysts, both which have various advantages disadvantages. In this research, Saccharomyces cerevisiae, biocatalyst, recombinant ho...

DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...

A sheep liver cDNA clone for the cytosolic serine hydroxymethyltransferase (SHMT) was isolated and its nucleotide sequence determined. The full-length cDNA of SHMT was placed under the control of T7 promoter in pET-3C plasmid and expressed in Escherichia coli. The overexpressed enzyme, present predominantly in the soluble fraction, was catalytically active. The recombinant SHMT was purified to ...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Raw starch degrading enzyme specially glucoamylase with binding domain (SBD) has great values in the processing industry because it digests particles below gelatinization temperature by releasing glucose from non-reducing ends sequentially. The purpose of study was to measure secretion levels recombinant Pichia pastoris, using α-mating factor signal peptide (α-MF) and native Aspergillus flavus ...

objectives: in present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. materials and methods: we have cloned bovine prochymosin gene in methylotrophic yeast, p. pastoris, using ppic9k as an expression vector. the recombinant plasmid was transformed into the host by electroporation, and it was expressed in optimum con­ditions...

visceral leishmaniasis (vl) is a zoonotic disease caused by leishmania species. dogs are considered to be the main reservoir of vl. a number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. however, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. a variety of serological tests ...

background: allergy is a clinical disorder affecting humans worldwide. allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the na...