The Sputnik V COVID-19 vaccine (Gam-COVID-Vac) is an adenoviral vector-based against the SARS-CoV-2 virus developed by Gamaleya National Research Centre for Epidemiology and Microbiology in Moscow, Russia. It consists of two recombinant adenovirus vectors; type 26 (rAd26) 5 (rAd5), with a heterologous approach used to reduce risk immune responses vector component. Findings from published studie...

Recombinant adenovirus administration gives rise to transgene-independent effects caused by the ability of the vector to activate innate immunity mechanisms. We show that recombinant adenoviruses encoding reporter genes trigger IFN-alpha and IFN-beta transcription from both plasmacytoid and myeloid mouse dendritic cells. Interestingly, IFN-beta and IFN-alpha5 are the predominant transcribed typ...

Recombinant interferon-γ (IFNγ) production in cultured lentivirus (LV) was explored for inhibition of target virus in cells co-infected with adenovirus type 5 (Ad5). The ability of three different promoters of CMV, EF1α and Ubiquitin initiating the enhanced green fluorescence protein (GFP) activities within lentiviruses was systematically assessed in various cell lines, which showed that certai...

Vaccination using recombinant tumor-derived T-cell antigen receptor (TCR) protein induces a protective, idiotype-specific immune response against a murine T-cell tumor. However, the technically demanding task of producing patient-specific, recombinant TCR protein restricts the translation of TCR vaccines for clinical use. We report here the development of an effective recombinant TCR adenovirus...

The aim of this study was to construct an adenoviral expression vector for vascular endothelium growth factor 121 (VEGF121)-FLAG and humanized Renilla reniformis green fluorescent protein (hrGFP-1) genes, and to observe their expressions in bone marrow mesenchymal stem cells. Using pTG19T-VEGF121 as a template, polymerase chain reaction technology was adopted to mutate the VEGF121 gene by remov...

The prevalence of Adenovirus was determined among children with gastroenteritis/ diarrhea in Warri, Southern, Nigeria. Adenovirus was detected using a lateral flow immunochromatographic test kit (Gastro Vir-Strip, Coris Bioconcept, Belgium). The prevalence of Adenovirus was 11 (14.7%) and there were no association with age and gender of subjects. There is need for routine screening of Adenoviru...

The EIa region of an Adenovirus 5 recombinant has been substituted by a modular gene encoding dihydrofolate reductase (DHFR). In this recombinant, the mouse DHFR cDNA was positioned behind sequences of the major late promoter and the complete tripartite leader. The leader sequences end in the normal 5' splice site (SS) of the third leader, so that RNA splicing joins the tripartite leader to a 3...

Human adenoviruses are non-enveloped, icosahedral viruses, approximately 130 nm in diameter. The linear, double-stranded DNA viral genome containing covalently attached terminal protein derived from a unique replication process is almost 36 Kb in length. Although forty nine serotypes of human adenovirus in six serological subgroups have been identified, only serotypes 2 and 5 of subgroup C have...

Joanne M. Wroblewski lez-Garcia M, Koedens M, Fukunga N, Davidson B, Dick J, Minn A, Boise L, Thompson C, Wicha M, Nunez G: A recombinant bclL. Travis Lay xs adenovirus selectively induces apoptosis in cancer cells but not Timothy C. Meeker in normal bone marrow cells. Proc Natl Acad Sci USA 92:11024, Markey Cancer Center 1995 University of Kentucky 4. Wroblewski J, Lay L, Van Zant G, Phillips ...