objective: in this study, a sybr green real-time rt-pcr assay for quantification of hiv-1 viral rna was developed. materials and methods: this assay was performed based on amplification of the pol region of hiv-1 and product analysis by an abi 7500 system. we quantified hiv-1 viral load in 26 seropositive patients by this system and the data were subsequently compared with results obtained wit...

background and aim: tinnitus is perception of sound in absence of external source. reaction time is the time between sensory stimuli and a behavior. alternation of auditory reaction time has been shown in the literature in patients with tinnitus. this study has investigated the auditory reaction time in quiet and noise to different frequencies. methods: fifteen subjects with chronic tinnitus pa...

During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in 310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conserve genes to search any BTV strains. Using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. Sensitivity of both molecular techniques evalua...

Real-time reverse transcription polymerase chain reaction (RT-PCR) is a commonly used technique to analyze gene expression. There has been little research conducted to test if SuperScript III quantitative one-step (reverse transcription carried out in the same tube as PCR) and two-step (reverse transcription carried out in a separate reaction) RT-PCR systems provide similar real-time results. I...

A newly developed real-time RT-polymerase chain reaction assay for quantitation of hepatitis C virus (HCV) RNA in human plasma and serum was applied. A pair of primers and a probe (molecular beacon) were designed that are specific for the recognition of a highly conservative 5'-non-coding region (5'-NCR) in HCV genome. HCV real-time RT-PCR assay had a sensitivity of 1000 RNA copies per reaction...