The objective of this study was to evaluate the occurrence of aflatoxin M1 (AFM1) in raw milk samples from 18 dairy farms in the Yangtze River Delta region during four different seasons. A total of 72 tank milk samples was collected with 18 samples for each season. Milk AFM1 was detected using LC-MS/MS. The AFM1 was detected in 43 milk samples (59.7%) ranging in concentration from 10 to 420 ng/...

A simple, rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in raw milk, milk products and animal feed. The limit of detection was estimated to be 0.05 μg/mL in raw milk, since the detection test line on the strip test completely disappeared at this concentration. The limit of detection was 2 μg/mL...

BACKGROUND Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. METHODS Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and v...

the suitability of milk preserved by refrigeration and co2 addition for the manufacture of plain yoghurt using two commercial strains of lactobacillus delbrueckii ssp. bulgaricus and streptoccocus thermophilus and probiotic yoghurt by lactobacillus acidophilus was evaluated. yoghurts (plain and probiotic) manufactured after milk pasteurization, from fresh and refrigerated co2 treated samples (p...

Mastitis is an inflammatory reaction of the mammary tissue in response to the introduction and multiplication of pathogens and is characterized by increasing somatic cell count (SCC) in milk. The main objective of present study is to assess the probable associations between somatic cell (SC) levels and calcium and phosphorus and ratio of Ca/P in raw milk. Milk samples were collected from indivi...

Consumption of raw cow's milk instead of industrially processed milk has been reported to protect children from developing asthma, allergies, and respiratory infections. Several heat-sensitive milk serum proteins have been implied in this effect though unbiased assessment of milk proteins in general is missing. The aim of this study was to compare the native milk serum proteome between raw cow'...

A method based on amino-modified silica-coated magnetic nanoparticles (ASMNPs) and polymerase chain reaction (PCR) was developed to rapidly and sensitively detect foodborne pathogens in raw milk. After optimizing parameters such as pH, temperature, and time, a trace amount of genomic DNA of pathogens could be extracted directly from complex matrices such as raw milk using ASMNPs. The magnetical...

Contrary to the safe practices of milk pasteurization or sterilization, which effectively reduce foodborne outbreaks incidence associated with raw milk and dairy products use, outbreaks caused by such products continue to occur. Despite this fact, a worldwide movement advocating for the rights of raw milk and cheese selling and consumption, due to their specific nutritive characteristics, has s...

materials and methods: a total of 285 commercial and traditional dairy products as well as 267 pasteurized and raw milk samples were collected during one year. the samples were studied by culturing and the positive-culture samples were investigated using pcr techniques. results: the results of culture showed that 52 (9.42%) and 28 (5.07%) of the total 552 milk and dairy samples were positive fo...