Protein translation is the most expensive operation in dividing cells from bacteria to humans. Therefore, managing the speed and allocation of resources is subject to tight control. From bacteria to humans, clusters of relatively rare tRNA codons at the N'-terminal of mRNAs have been implicated in attenuating the process of ribosome allocation, and consequently the translation rate in a broad r...

An expression vector containing the gene encoding most common isoform Bet v 1.0101 of 1 protein, major birch pollen allergen, was created for a subsequent in prokaryotic system Escherichia coli. Total RNA from collected Belarus used as matrix. The pJC40-Веt obtained using molecular-genetic methods: cloning, ligation, transformation. specificity cloned fragment confirmed by sequencing. During st...

Introduction: Endometriosis is a prevalent gynecological disorder among women which is diagnosed by the growth of endometrial tissue outside of uterus and is mainly accompanied by severe pelvic pain and infertility. P53 also known as cellular tumor antigen P53 inside codons 11, 72 and 248 are contained with single nucleotide changes in which tends to be nearly rampant.This will probably b...

The presence of clusters of rare codons is known to negatively impact the efficiency and accuracy of protein production. In this paper, we demonstrate a statistical method of identifying such clusters in the coding sequence of a gene. Using E. coli as our model organism, we show that genes having denser clusters tend to have lower protein yields.

The codon preference plot is useful for locating genes in sequenced DNA, predicting the relative level of their expression and for detecting DNA sequencing errors resulting in the insertion or deletion of bases within a coding sequence. The three possible reading frames are displayed in parallel along with the open reading frames and plots of the location of rare codons in each reading frame.

Patterns of codon bias in Drosophila suggest that silent mutations can be classified into two types: unpreferred (slightly deleterious) and preferred (slightly beneficial). Results of previous analyses of polymorphism and divergence in Drosophila simulans were interpreted as supporting a mutation-selection-drift model in which slightly deleterious, silent mutants make significantly greater cont...

DNA codons, consisting of triplet nucleotides (NTs), could play important roles for RNA transcription and protein translation in living systems. Therefore, their recognition be seen diagnosis therapy purposes. Based on sequence formations Adenine (A), Guanine (G), Cytosine (C) Thymine (T) NTs, 64 codons were investigated this work regarding complexation with a molecular cubane (CUB) wire. To ac...