Two hundred and eighty Rapid Plasma Reagin (RPR) positive sera with an emphasis on cases with negative and borderline positive Treponema pallidum haemagglutination assay (TPHA) results were selected. Modified TPHA (M-TPHA) and fluorescent treponemal antibody absorption (FTA-abs) tests were used for comparison. One hundred and twenty five samples were TPHA negative, of which 78 and 69 cases were...

A procedure is described for the routine laboratory diagnosis of viral serum antibodies. Antigens are dotted on nitrocellulose strips or sheets, and sera are applied on absorbent paper strips. Antigen-antibody complexes are detected with enzyme-conjugated antiglobulin and development of a colored, insoluble substrate product. The test allows processing of multiple sera in one 3- to 5-h operatio...

A simple and rapid immunoassay of morphine based on latex agglutination inhibition reaction has been developed. Morphine-6-succinate is a morphine derivative, and its bovine serum albumin (BSA) conjugates were synthesized and characterized. The hapten density in the conjugate was determined spectroscopically to be 8.2 mol per mol of BSA. It was found that morphine-6-succinyl-BSA (M-6-S-BSA) exh...

This paper reports on the development of a prototype solution for tracking and monitoring shipping containers. Deploying wireless sensor networks (WSNs) in an operational environment remains a challenging task. We strongly believe that standardized methodologies and tools could enhance future WSN deployments and enable rapid prototype development. Therefore, we choose to use a step-by-step appr...

Seroreactivity of sera from 109 patients with first-infection primary syphilis was 98.2% in the fluorescent treponemal antibody absorption test, 92.7% in the rapid plasma reagin 18-mm circle card test, 72.5% in the microhemagglutination test (MHA-TP), and 72.5% in the Venereal Disease Research Laboratory test. Seroreactivity of sera from 18 patients with primary syphilis with documented previou...

Rapid and easy determination of protective neutralization antibody (NAb) against rabies in the field is very important for an early and effective response to rabies in both animal and human health sectors. The rapid neutralizing antibody detection test (RAPINA), first developed in 2009 and then improved in 2012, is a quick test allowing detection of 0.5 IU/ml antibodies in human and animal sera...