results the results revealed the formation of spherical silver nanoparticles with an average size of 10 nm. moreover, concentration of snps in a 25 ml sample containing both snps and plant extract biomass was 2.934 mg/l on average. serial microdilution test showed that snps at the concentration of 50 μg/ml can inhibit growth of the pathogen. conclusions the present study extends the existing li...

in the present study, four carbon sources were tested for the polyester synthesis of pseudomonas aeruginosa pa01 which is a ubiquitous environmental bacterium and is one of the top three causes of opportunistic human infections. these included linear c8 to c11 carboxylic acids. octanoic acid, nonanoic acid, decanoic acid and undecanoic acid were added to m1 minimal medium to final concentration...

Phages possess the ability to selectively eliminate pathogenic bacteria by recognizing bacterial surface receptors. Since their discovery, phages have been recognized for potent bactericidal properties, making them a promising alternative antibiotics in context of rising antibiotic resistance. However, rapid emergence phage-resistant strains (generally involving temperature phage) and limited h...

in the present study, a simple, efficient and fast synthetic strategy was reported for the green biosynthesis of silver nanostructures (i.e. nanoroads and nanoparticles) by the extract of suaeda acuminata plant, without any catalyst, template or surfactant. aqueous extracts were obtained by maceration and microwave assisted extraction (mae) methods. in mae procedure, the effect of microwave pow...

nanomaterials (nms) are widely used in health and biomedical applications, however, only a few studies investigate their toxic effects.  the present report signifies a contribution to the study of the toxic effects of silver nanoparticles (ag nps)  on   e. coli cells, which is a model organism of anthropogenic pollution. the toxicity of nanoparticles depends on their  chemical and surface prope...

background and objective: early determination of antibacterial susceptibility increases the success of therapy, decreases unnecessary use of antibacterial agents and side-effects, and lowers the overall healthcare cost. materials and methods: a rapid colorimetric method for antibacterial susceptibility testing named qui-sensitest (inovative biotechnology organization, istanbul, turkey) was eval...

With the rapid development of synthetic biology, production 2′-fucosyllactose by biological fermentation gradually has basis for industrialization. However, lack efficient downstream technology fermentation, especially purification technology, become main factor limiting its commercialization. In this study, based on general E. coli biosynthesis broth, most impurities were removed and concentra...

The Lipid A moiety of endotoxin potently activates TLR-4 dependent host innate immune responses. We demonstrate that Lipid-A mediated leukotriene biosynthesis regulates pathogen-associated molecular patterns (PAMP)-dependent macrophage activation. Stimulation of murine macrophages (RAW264.7) with E. coli 0111:B4 endotoxin (LPS) or Kdo2-lipid A (Lipid A) induced inflammation and Lipid A was suff...

Cancer cells frequently exhibit specific alterations in their metabolic activity. This metabolic reprogramming supports the increased production of metabolic intermediates for the synthesis of proteins, nucleic acids and lipids, and is a prerequisite for the rapid proliferation of cancer cells. The most prominent metabolic alterations in cancer are an increase in glucose uptake and the use of a...

In response to elicitors in the oral secretions of caterpillars, plants produce and release volatile chemicals that attract predators and parasitoids of the caterpillar while it feeds. The most prevalent elicitors are fatty acid amides consisting of 18-carbon polyunsaturated fatty acids coupled with l-glutamine. We demonstrate rapid CoA- and ATP-independent in vitro biosynthesis of the fatty ac...