PREMISE OF THE STUDY Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged ...

During an investigation of antitumor substances from Nigella glandulifera Freyn et Sint. (Ranunculaceae) the cytotoxicity of two oleanane triterpene saponins isolated from the seeds of this species, kalopanaxsaponins A and I, was evaluated against HepG2, drug resistant HepG2 (R-HepG2) (two hepatocyte cell lines) and primary cultured normal mouse hepatocytes. Evident cytotoxic activities were ob...

Clematis pseudotubulosa (Ranunculaceae), a new species from Gyeonggi-do in the Republic of Korea, is described and illustrated. The morphologically similar to C. takedana but differs its leaves, which are always ternate with shallowly lobed leaflets (vs. 1-pinnate deeply three five leaflets), oblong-shaped flower buds ovoid), larger flowers smaller flowers), pantoporate pollen tricolpate). A de...

Chronic treatment with Delphinium denudatum (Dd) (Jadwar) (family: Ranunculaceae, 200-1600 mg/kg) suppressed morphine withdrawal jumps in a dose-dependent manner, a sign of the development of dependence to opiate as assessed by naloxone (2 mg/kg) precipitation withdrawal on day 10 of testing in mice. Repeated administration of Dd (200-1600 mg/kg) for 9 days attenuated the development of toleran...