We performed dengue virus (DENV) serology and quantitative real-time pan-DENV reverse transcription-PCR (RT-PCR) on 186 sera of 171 patients returning from the tropics. DENV loads significantly decreased with increasing times of disease and were higher in immunoglobulin M-negative samples. In the first week of disease, pan-DENV RT-PCR is the test of choice.

Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR). A nanofluidic real-time PCR system was used to develop novel high-throughput methods for qualitative molecular detection (RT-qPCR array) and quantification of human pathogeni...

The use of molecular techniques such as quantitative RT-PCR depends on the quality of cellular RNA. In particular, RNA extraction from endoscopic biopsies is difficult with respect to yield, and especially integrity. Endoscopic biopsies taken from the gastric antrum, corpus and duodenum were subjected to various RNA extraction protocols, and the RNA was used for quantitative RT-PCR. The subsequ...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

Polymerase chain reaction (PCR) is a very powerful tool for qualitative evaluation of nucleic acids due to its high efficiency and convenience. Together with the reverse transcription (RT) reaction, the PCR method has been widely applied to the quantitative measurement of DNA and RNA messages. Since RT-PCR is much more sensitive than all of the traditional methods for quantification of mRNA, in...

Quantitative reverse transcription PCR (RT-PCR) is typically used to assay transcript abundance (often generalized as “gene expression”) by measuring a specific cDNA level. The method is very sensitive and is suitable for a broad range of cDNA concentrations. Its reliability depends on, among other factors, appropriate normalization (for a review, see References 1 and 2). The preferred method o...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 6 751–762 www.jcb.org/cgi/doi/10.1083/jcb.201312062 JCB 751 Correspondence to Kota Saito: [email protected] Abbreviations used in this paper: BFA, Brefeldin A; CT, C terminus; ERGIC, ER– Golgi intermediate compartment; GEF, guanine-nucleotide exchange factor; MBP, maltose-binding protein; qRT-PCR, quantitative RT-PCR; R...

PURPOSE Molecular analysis of melanoma sentinel nodes (SN) is sensitive, but poorly specific because metastases cannot be distinguished from benign nevus inclusions (BNI). We investigated whether quantitative reverse transcription-PCR (RT-PCR) detection of MART-1 and tyrosinase mRNAs could improve this specificity and contribute to SN assessment. EXPERIMENTAL DESIGN Two hundred twenty SNs fro...