In cancer cells, the aberrant conversion of pyruvate into lactate instead of acetyl-CoA in the presence of oxygen is known as the Warburg effect. The consequences and mechanisms of this metabolic peculiarity are incompletely understood. Here we report that p53 status is a key determinant of the Warburg effect. Wild-type p53 expression decreased levels of pyruvate dehydrogenase kinase-2 (Pdk2) a...

the aim of this study was to determine the short-term (1 session) and long-term (36 sessions) effects of various exercise training (endurance, sprint, and combined) on plasmatic indexes of muscle damages (lactate dehydrogenase, creatine kinase, and malondialdehyde). for this purpose, 40 young rats (3 months old) were randomly divided into four groups including control, endurance, sprint, and co...

The regulation of the pyruvate dehydrogenase multienzyme complex was studied in the isolated perfused rat heart. Because 95% of the “CO2 produced as a result of the decarboxylation of [l-‘4C]pyruvate occurs via pyruvate dehydrogenase in the heart, the rate of 14COz production from infused [l-‘4C]pyruvate was utilized as the monitor of the metabolic flux through this enzyme complex. In contrast ...

The adaptation of mitochondrial ATP production rate (MAPR) to training and detraining was evaluated in nine healthy men. Muscle samples (approximately 60 mg) were obtained before and after 6 wk of endurance training and after 3 wk of detraining. MAPR was measured in isolated mitochondria by a bioluminometric method. In addition, the activities of mitochondrial and glycolytic enzymes were determ...

The regulation of the pyruvate dehydrogenase multienzyme complex was studied in the isolated perfused rat heart. Because 95% of the “CO2 produced as a result of the decarboxylation of [l-‘4C]pyruvate occurs via pyruvate dehydrogenase in the heart, the rate of 14COz production from infused [l-‘4C]pyruvate was utilized as the monitor of the metabolic flux through this enzyme complex. In contrast ...

In both colon cancer and type 2 diabetes, metabolic changes induced by upregulation of the Wnt/beta-catenin signaling and downregulation of peroxisome proliferator-activated receptor gamma (PPAR gamma) may help account for the frequent association of these two diseases. In both diseases, PPAR gamma is downregulated while the canonical Wnt/beta-catenin pathway is upregulated. In colon cancer, up...

Pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and ADP to yield ATP and Pyruvate. Geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. Given that limited proteolysis experiments can be successfully used to probe conformational features of p...

PURPOSE High-fat diets (HFD) feeding is an important risk factor for obesity that is accompanied with metabolic syndrome. Appropriate exercise is recommended for obesity prevention. The molecular mechanisms and cellular pathways activated in response to HFD and exercise are not well understood. The purpose of this study was to investigate the effect of 8 weeks endurance training on some plasma ...

The phylogeny and substitution rates of the mammalian X chromosome-located and autosomal phosphoglycerate kinase and pyruvate dehydrogenase genes were investigated. Compatibility analysis was used to show reticulate evolution in these genes. Analysis of the marsupial, mouse, and human phosphoglycerate kinase genes suggests that at least two recombination events have taken place, one occurring a...