The glycine betaine (betaine), interacts with several types of proteins with diverse structures in vivo, and in the contact regions, the aromatic rings of protein residues are frequently found beside the trimethylammonium group of betaine, implying the importance of the cation−π interactions in recognition of this molecule. The crystal structures determined by X-ray crystallography of the compl...

The encapsulation of chloroperoxidase from Caldariomyces fumago (CPO) in block copolymer polymersomes is reported. Fluorescence and electron microscopy show that when the encapsulating conditions favour self-assembly of the block copolymer, the enzyme is incorporated with concentrations that are 50 times higher than the enzyme concentration before encapsulation. The oxidation of two substrates ...

This work deals with the use of pruning ANNs in conjunction with genetic algorithms for resolving nonlinear multicomponent systems based on oscillating chemical reactions. The singular analytical response provides by this chemical system after its perturbation was tted to a gaussian curve by least-square regression and the estimates were used as inputs to the ANNs. The proposed methodology was ...

a kinetic spectrophotometric method for rapid measurement of thiocyanate in serum, urine and sewage water with no need for removed of interfering substances in described. the method is based on the inhibitory effect of thiocyanate on the oxidation of pyrogallol red by bromated and nitrite which is monitored at 467 nm. the variables affecting the rate of the reaction were investigated and the op...

18O labeling studies on the catechol 1,2-dioxygenase-catalyzed oxidative cleavage of pyrogallol demonstrate that the enzyme functions both as a dioxygenase and a monooxygenase in this reaction. Two products are observed, 2-pyrone-6-carboxylic acid, 99% singly labeled at the carboxylate, and 2-hydroxy-cis,cis-muconic acid, 74% doubly labeled (one 18O at each carboxylate) and 24% single labeled (...

The measurement of urinary total protein continues to have a place in the repertoire of clinical laboratory tests, particularly in the clinical assessment of patients with renal disease. Although the test volume is often modest, there exists the need to perform the assay on the major automated chemistry analyzers currently in use in most hospital laboratories. We have evaluated the performance ...