Aiming for hydrogen and oxidation easily produced in the melting process of Al-Mg alloys, three processes, including non-vacuum static melt treatment, rotary-injection purification vacuum-stirring purification, were used to purify Al-6Mg alloy melt. The content inclusion studied by means solid-state measurement method, reduced pressure test FESEM EDS. results show that effect is better than tre...

Abstract Background As wild poliovirus is eradicated, preventing circulation of vaccine-derived top priority. Our lab developed real-time multiplex PCR assays and deep sequencing methodology to detect characterize OPV strains from stool samples. The method requires gel purification product created viral RNA in However, the process filters out a significant portion Here, we compare gel- vs. DNA-...

Culture medium used in antibody purification contains growth factors, but is usually discarded after antibody purification. We examined to determine whether the growth factors can be (i) recovered and (ii) used in subsequent cell culture. Using this medium enhanced the survival of hybridoma cells and improved antibody production. Culture medium previously used in antibody purification offers an...

We achieved the synthesis of important medronic acid monoalkyl esters via the dealkylation of mixed trimethyl monoalkyl esters of medronic acid. Two methods were developed for the purification of medronic acid monoesters: 1) small scale (10-20 mg) purification by using hydroxyapatite and 2) large scale (tested up to 140 mg) purification by high-performance countercurrent chromatography (HPCCC).

BACKGROUND p-aminobenzamidine (p-ABA) is used as a ligand in the purification of many serine proteases and in their removal from heterogeneous samples. Moreover, p-ABA has a potent ability to bind Ca(2+)-binding proteins. The binding ability and use of p-ABA in purification processes is still not fully understood. METHODOLOGY/PRINCIPAL FINDINGS A p-Aminobenzamidine (p-ABA) ligand enabled the ...

We have partially purified human estrogen receptor from MCF-7 breast cancer cells in order to further characterize the chemical and biological properties of the receptor and to prepare specific receptor antibodies for radioimmunoassay. The estrogen receptor from MCF-7 cell cytosol was purified 1,166-fold (27% recovery) over starting cytosol by combining ammonium sulfate precipitation, affinity ...

ABSTRACT It is known that various plants have purification ability for indoor air pollutants. In this paper the purification mechanism of foliage plant was investigated by analyzing bioelectrical potential. It was first shown that the air purification ability of a plant depends on various environmental conditions, especially on light irradiation. It was then clarified that the bioelectrical pot...

Cellulose is a renewable and biodegradable natural biopolymersynthesized mainly in plants such as wood, bark, cotton fiber, green algae, some bacteria and animals. Pre-treatment processes such as alkaline purification and enzymatic purification are applied in order to create a proper sorption characteristic of regenerated cellulose fiber. Prior to the application of cellulose/cellulose derivati...

We present and demonstrate a novel technique for the purification of nucleic acids from biological samples using isotachophoresis (ITP). We demonstrate a simple and rapid method to achieve ITP-based extraction, preconcentration, and purification of DNA from nanoliter volumes of whole blood. We show that ITP purification yields genomic DNA samples which can be quantitated with fluorescence measu...