The nucleotide sequence of a 3,202-base-pair fragment which contained the minimum region required for replication of the broad-host-range plasmid, pTF-FC2, has been determined. At least five open reading frames and a region that affected the host range were identified. Proteins corresponding in size and location to four of the five open reading frames were produced in an in vitro transcription-...

Stem Leydig cells (SLCs), located in the testicular interstitial compartment in the mammalian testes, are capable of differentiating to testosterone-synthesizing Leydig cells (LCs), thus providing a new strategy for treating testosterone deficiency. However, no previous reports have identified and cultured SLCs derived from the pig. The aim of the current study was to isolate, identify, and cul...

A recent study has shown that the double-pass method provides a good estimate of the ocular modulation transfer function (MTF) but that it does not yield the phase transfer function (PTF) [J. Opt. Soc. Am. A 12, 195 (1995)]. Therefore, one cannot recover the true retinal point-spread function (PSF). We present a modification of the double-pass method to overcome this problem. The key is to brea...

Seasonal wetlands in the tropics are important habitats for local and migratory bird species. In northwestern Pacific of Costa Rica, Palo Verde National Park has one most seasonal Central America. The management history this wetland shown impact invasive plant species such as Parkinsonia aculeata L. whose cover extension canopy structure not only ecological niches species, but also hydrology. A...

The aim of this study was to compare the ability of electro- and echocardiography to detect enlargement of the left atrium. Seventy-four patients, divided into three groups (eighteen normal, thirty-six valvular disease, twenty hypertension and/or coronary artery disease) were studied. The P wave terminal force in lead V1 (PTF-V1) was measured from a standard 12 lead electrocardiogram, and the i...

Atterberg limits are broadly used for engineering and geology purposes as well in agricultural environmental applications. Laboratory methods their determination are, however, laborious, destructive tool dependent. The aim of this study was to test the feasibility using visible near-infrared spectroscopy (vis–NIRS) a fast accurate alternative conventional measurements (LL PL) PI 229 geographica...