An effi cient whole plant regeneration method from callus cultures of Piper auritum was achieved through organogenesis derived from leaf tissue. Proliferating callus and shoot cultures derived from leaf tissue explants placed on Murashige and Skoog (MS) medium supplemented with 2.0 mg·L 2, 4-dichlorophenoxyacetic acid (2,4-D) plus 1.5 mg·L kinetin. Optimum combination of hormones (mg·L) for sho...