Previous research showed that the heme-requiring human pathogen Haemophilus influenzae lacks the first six of the seven enzymes required for heme synthesis, starting with the precursor, 5-amino levulinic acid. In this study, I demonstrated either directly or by reasonable inference that all 57 strains of H. influenzae examined, including 2 unable to grow on protoporphyrin IX, possess ferrochela...

We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct determination of protoporphyrin in the same samples. In the direct assay the f...

Butafenacil is an herbicide that inhibits protoporphyrinogen oxidase (PPOX), an enzyme that catalyzes oxidation of protoporphyrinogen IX to protoporphyrin IX during chlorophyll and heme biosynthesis. Based on a high-content screen, we previously identified butafenacil as a potent inducer of anemia in zebrafish embryos. Therefore, the objective of this study was to begin investigating the utilit...

Peter Böger and Roswitha M iller Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-78434 Konstanz, Bundesrepublik Deutschland Z. Naturforsch. 49c, 775-780 (1994); received October 13, 1994 Peroxidizing Herbicides, Safeners, Protoporphyrin IX Accumulation, Protoporphyrinogen Oxidase, Mixed Function Oxidase A number of safeners like naphthalene-1,8-dicarboxylic acid an...

Magnesium protoporphyrin IX methyltransferase (ChlM), catalyses the methylation of magnesium protoporphyrin IX (MgP) at the C(6) propionate side chain to form magnesium protoporphyrin IX monomethylester (MgPME). Threading methods biased by sequence similarity and predicted secondary structure have been used to assign this enzyme to a particular class of S-adenosyl-L-methionine (SAM)-binding pro...

A panel of 30 previously characterized strains representing five genomovars from the Burkholderia cepacia complex (E. Mahenthiralingam, T. Coenye, J. W. Chung, D. P. Speert, J. R. W. Govan, P. Taylor, and P. Vandamme, J. Clin. Microbiol. 38:910--913, 2000) were examined for their iron protoporphyrin IX-binding ability. These included B. cepacia genomovars I and III and B. stabilis (formerly B. ...

The role of hemin in the maintenance of protein synthesis in reticulocyte lysates was examined by comparing the effects of various porphyrins and metalloporphyrins on the protein kinase activity of the hemin-controlled repressor and on protein synthesis. The porphyrin requirements for maintenance of protein synthesis were relatively specific. Iron and cobalt metalloporphyrins sustained protein ...