Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps surfaces, with the aim identifying surface markers epitopes diagnostic therapeutic applications. The analysis two cancer lines, HN5 MDA-MB-468, described herein as proof concept, along selective targeting...

because of the huge amounts of proteomic data and demand for new methods of laboratory analysis results, proteins collective analysis, in addition to taking less time, biostatistician assist at identification of new patterns in the data set. in this study, rat hippocampus proteome in normal and alzheimer's disease (ad) were analyzed by using proteomic techniques and bioinformatics’ analysis. pr...

Recent advancement in mass spectrometry leads us to a new era of proteomic analysis. Human saliva can be easily collected; however, the complexity of the salivary proteome in the past prevented the use of saliva for proteomic analysis. Here we review the development of proteomic analyses for human saliva and focus on the use of a new mass spectrometric technology known as surface-enhanced laser...

Although human DNA sequences have already been successfully decoded, there is still much we do not understand about the pathogenesis of human diseases. Proteomic analysis is comprehensive analysis of proteins, and there is a high probability that recent advances in this field will enable its application to individualized therapy based on thorough understanding of the pathogenesis of human disea...

Advances in proteomic technologies have led to the creation of large-scale proteome databases that can be used to elucidate invaluable information on the dynamics of the metabolic, signaling and regulatory networks and to aid understanding of physiological changes. In particular, proteomics can have practical applications, for example, through the identification of proteins that may be potentia...

Plumbagin (PLB) has exhibited a potent anticancer effect in preclinical studies, but the molecular interactome remains elusive. This study aimed to compare the quantitative proteomic responses to PLB treatment in human prostate cancer PC-3 and DU145 cells using the approach of stable-isotope labeling by amino acids in cell culture (SILAC). The data were finally validated using Western blot assa...

Human saliva has great potential for clinical disease diagnostics. Constructing a comprehensive catalogue of saliva proteins using proteomic approaches is a necessary first step to identifying potential protein biomarkers of disease. However, because of the challenge presented in cataloguing saliva proteins with widely varying abundance, new proteomic approaches are needed. To this end, we used...

Proteomic profiling of Sengers patient fibroblasts and AGK knockout models identifies remodeling the mitochondrial proteome, including one-carbon metabolism enzymes, inner membrane serine transporters, sideroflexins, Complex I subunits assembly factors.