efficiency of enzymes which are used in industrial or environmental applications is highly dependent on their thermal stability. in this study, the stability of dfpase has been evaluated after introducing disulfide bonds to the structure. the results obtained from a series of protein design software were subjected to molecular dynamics simulation at different temperature to test the performance...

ESI-MS has been used extensively over the last decade to study protein-protein interactions [1]. Serine proteases and their inhibitors provide a valuable model for the study of protein complexes as a great deal is known about solution binding and about X-ray crystal structures of such systems. It is generally accepted that hydrophobic interactions are weakened in the gas phase and that hydrophi...

introduction: proteins are food ingredients with critical functional properties and participation in developing food products. so far, functional properties of several plant proteins such as pea, chickpea and lentil, groundnut, beach pea and bayberry have been investigated. nowadays, there is an increasing demand for plant proteins because they are available and inexpensive. legume proteins are...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

clostridium botulinum type a (bont/a) produces a neurotoxin recently found to be useful as an injectable drug for the treatment of abnormal muscle contractions. the catalytic domain of this toxin which is responsible for the main toxin activity is a zinc metalloprotease that inhibits the release of neurotransmitter mediators in neuromuscular junctions. a cell penetrating cationic peptide, tat, ...